Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis
Direct Imaging of Intracellular Signaling Components That Regulate Bacterial Chemotaxis
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DOI：
10.1126/scisignal.2004963
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发表时间：
2014-04-01
影响因子：
7.3
通讯作者：
Ishijima, Akihiko
中科院分区：
文献类型：
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作者：
Fukuoka, Hajime;Sagawa, Takashi;Ishijima, Akihiko
细菌趋化系统通过细胞内信号分子Chey的磷酸化形式(Chey-P)来调节鞭毛马达的旋转方向。Chey-P与马达的结合被认为会将马达的旋转方向从逆时针转换为顺时针。我们通过在活细胞中同时显示绿色荧光蛋白标记的Chey和电机的旋转开关，证明了Chey-P的结合和解离直接调节了电机的旋转开关。13+/-7个Chey-P分子的结合足以诱导顺时针旋转，在切换过程中，Chey-P分子在类似于100ms的时间内与马达结合并解离。因此，我们直接测量了细胞内信号蛋白对信号转导途径输出的调节。
The bacterial chemotaxis system regulates the rotational direction of flagellar motors through an intracellular signaling molecule, the phosphorylated form of CheY (CheY-P). The binding of CheY-P to a motor is believed to switch the motor's rotational direction from counterclockwise to clockwise. We demonstrated that the rotational switch of a motor was directly regulated by the binding and dissociation of CheY-P by simultaneously visualizing CheY tagged with green fluorescent protein and the rotational switching of a motor in live cells. The binding of 13 +/- 7 CheY-P molecules was sufficient to induce clockwise rotation, and CheY-P molecules bound to and dissociated from a motor within similar to 100 ms during switching. Thus, we have directly measured the regulation of the output from a signal transduction pathway by intracellular signaling proteins.