Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology.
Optimization of growth media for obtaining high-cell density cultures of halophilic archaea (family Halobacteriaceae) by response surface methodology.
复制标题
通过响应面法优化生长培养基，以获得嗜盐古菌（盐杆菌科）的高细胞密度培养物。
DOI：
10.1016/j.biortech.2009.01.033
复制
发表时间：
2009
影响因子：
11.4
通讯作者：
V. Kannan
中科院分区：
文献类型：
--
作者：
M. Manikandan;L. Pasic;V. Kannan
使用响应面法对盐杆菌 VKMM 013 的生长和生物量生产的培养基成分进行优化。估计二阶二次模型并根据模型生成的二次回归方程确定媒体成分。它们是营养肉汤中的 6.35gL−1 KCl、9.70gL−1 MgSO4、13.38gL−1 明胶和 12.00gL−1 可溶性淀粉，并补充有含有 20%（w/v）NaCl 的人造海水。在这些最佳条件下，获得的细胞浓度为0.746gL−1干重，与预测的细胞浓度一致。优化后的培养基显着缩短了细胞培养达到稳定期所需的时间，同时使生物量产量增加了近 2.4 倍。此外，在其他三种嗜盐古菌的细胞培养物中，使用优化的培养基可提高生长速度并提供高细胞密度。
Optimization of media components for the growth and biomass production of Halobacterium salinarum VKMM 013 was carried out using response surface methodology. A second order quadratic model was estimated and media components were determined based on quadratic regression equation generated by model. These were 6.35gL−1of KCl, 9.70gL−1of MgSO4, 13.38gL−1of gelatin and 12.00gL−1of soluble starch in nutrient broth supplemented with artificial seawater with 20% (w/v) of NaCl. In these optimal conditions, the obtained cell concentration of 0.746gL−1dry weight was in agreement with the predicted cell concentration. The optimized media significantly shortened the time required for cell culture to reach the stationary phase while providing a nearly 2.4-fold increase in biomass production. Furthermore, in cell cultures of three other halophilic archaea the use of optimized media enhanced growth rate and provided high-cell density.