Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria
Monitoring of gene expression in Fibrobacter succinogenes S85 under the co-culture with non-fibrolytic ruminal bacteria
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DOI：
10.1007/s00203-014-1049-0
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发表时间：
2015-03-01
影响因子：
2.8
通讯作者：
Kobayashi, Yasuo
中科院分区：
文献类型：
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作者：
Fukuma, Naoki M.;Koike, Satoshi;Kobayashi, Yasuo
产琥珀酸纤维杆菌是瘤胃中最重要的纤维分解菌之一。在以前的研究中，我们证实了在F。琥珀酸单胞菌S85与非纤维分解瘤胃菌株R-25和/或反刍月形单胞菌S137。本研究采用基因组测序的方法，对F.通过实时RT-PCR监测琥珀酸单胞菌S85。F.琥珀酸单胞菌S85在与非纤维溶解剂的单培养和共培养中是相似的。而F.琥珀酸单胞菌S85在共培养组中的表达显著高于单培养组（P < 0.01）。这一发现表明，F。与菌株R-25和/或S.反刍动物S137。富马酸还原酶和糖苷水解酶基因的mRNA表达量在F.将产琥珀酸菌S85与非纤维溶解剂共培养。这些结果表明F. succinogenes S85在S. ruminantium和R-25菌株。
Fibrobacter succinogenes is one of the most pivotal fibrolytic bacterial species in the rumen. In a previous study, we confirmed enhancement of fiber digestion in a co-culture of F. succinogenes S85 with non-fibrolytic ruminal strains R-25 and/or Selenomonas ruminantium S137. In the present study, mRNA expression level of selected functional genes in the genome of F. succinogenes S85 was monitored by real-time RT-PCR. Growth profile of F. succinogenes S85 was similar in both the monoculture and co-cultures with non-fibrolytics. However, expression of 16S rRNA gene of F. succinogenes S85 in the co-culture was higher (P < 0.01) than that of the monoculture. This finding suggests that metabolic activity of F. succinogenes S85 was enhanced by coexistence with strains R-25 and/or S. ruminantium S137. The mRNA expression of fumarate reductase and glycoside hydrolase genes was up-regulated (P < 0.01) when F. succinogenes S85 was co-cultured with non-fibrolytics. These results indicate the enhancement of succinate production and fiber hydrolysis by F. succinogenes S85 in co-cultures of S. ruminantium and R-25 strains.