Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis.
Molecular characterization and expression pattern of taurine transporter in zebrafish during embryogenesis.
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DOI：
10.1016/j.lfs.2008.02.015
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发表时间：
2008-05
期刊：
影响因子：
6.1
通讯作者：
D. Kozlowski;Zhong Chen;Lina Zhuang;Y. Fei;S. Navarre;V. Ganapathy
中科院分区：
文献类型：
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作者：
D. Kozlowski;Zhong Chen;Lina Zhuang;Y. Fei;S. Navarre;V. Ganapathy
牛磺酸及其转运蛋白(TAUT)在植入前胚胎中表达，但其在胚胎发生中的作用尚不清楚。为了研究Taut在胚胎发育中的作用，我们克隆了Taut斑马鱼，并对其进行了功能鉴定。斑马鱼taut基因编码625个氨基酸的蛋白质，与哺乳动物taut高度同源。当在哺乳动物细胞中表达时，斑马鱼TAUT以Na+/Cl−依赖的方式调节牛磺酸的摄取，其化学计量比为2+：CL−：牛磺酸。在斑马鱼胚胎中，牛磺酸和TauT基因在早期卵裂阶段存在，表明转运蛋白及其底物都是母源的。在胚胎发育过程中，TauT基因在视网膜、脑、心脏、肾脏和血管中有明显的合子表达。反义吗啉寡核苷酸抑制TRACT导致中枢神经系统细胞死亡和死亡率增加。这些发现表明，Taut在脊椎动物的发育过程中起着特殊的作用。
Taurine and its transporter (TauT) are expressed in preimplantation embryos, but their role in embryogenesis is not known. To investigate the role of TauT during embryonic development, we cloned and functionally characterized the zebrafish TauT. The zebrafish TauT cDNA codes for a protein of 625 amino acids which is highly homologous to mammalian TauT. When expressed in mammalian cells, zebrafish TauT mediates taurine uptake in a Na+/Cl−-dependent manner with a Na+:Cl−:taurine stoichiometry of 2:1:1. In the zebrafish embryo, taurine and TauT mRNA are present during early cleavage stages, indicating that both the transporter and its substrate are maternally derived. During embryogenesis, zygotic expression of TauT mRNA is evident in the retina, brain, heart, kidney, and blood vessels. Knockdown of TauT by antisense morpholino oligonucleotides leads to cell death in the central nervous system and increased mortality. These findings suggest a specific role for TauT during development in vertebrates.