Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection
Identification of a novel N4BP1-like gene from grass carp (Ctenopharyngodon idella) in response to GCRV infection
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DOI：
10.1016/j.fsi.2013.11.003
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发表时间：
2014-01-01
影响因子：
4.7
通讯作者：
Jian, Jichang
中科院分区：
文献类型：
--
作者：
Cai, Jia;Yang, Lin;Jian, Jichang
Nedd4结合蛋白1(N4BP1)是一种相互作用蛋白，也是Nedd4 E3连接酶的底物。然而，关于N4BP1的S功能的报道是有限的。本研究从草鱼(Ctenophyngodon Idella)中克隆了一个新的N4BP1基因(CiN4BP1)。CiN4BP1的全长cDNA序列(3022bp)包括一个2565bp的开放阅读框(ORF)，编码854个氨基酸的多肽，包含一个KH结构域和一个NYN结构域。该基因与水稻N4BP1基因同源性很高，同源性为47%。皮肤、鳃、头肾和脾中CiN4BP1基因的mRNA表达水平较高。草鱼呼肠孤病毒(GCRV)感染后，CiN4BP1在体内和体外均表达上调。此外，在CIK细胞中过表达CiN4BP1抑制了病毒基因的转录。提示CiN4BP1可能在病毒入侵的免疫应答中发挥重要作用。(C)爱思唯尔有限公司出版的2013年。
Nedd4 binding protein 1 (N4BP1) has been identified as an interacting protein and a substrate of Nedd4 E3 ligase. However, the report about N4BP1's function is limit. In this study, a novel N4BP1 gene (CiN4BP1) was cloned from grass carp (Ctenopharyngodon idella). The full-length cDNA sequence of CiN4BP1 (3022 bp) included an open reading frame (ORF) of 2565 bp, which encoded a putative peptides of 854 amino acids containing one KH domain and one NYN domain. It was close homology (47% identify) to Oryzias latipes N4BP1. And mRNA expression of CiN4BP1 gene showed relatively high level in skin, gill, head kidney and spleen. After grass carp reovirus (GCRV) infection, CiN4BP1 was up-regulated in vivo and in vitro. Furthermore, overexpression of CiN4BP1 in CIK cells inhibited viral gene transcription. These data indicated that CiN4BP1 might play an important role in immune response to viral invasion. (C) 2013 Published by Elsevier Ltd.