CRYOPRESERVATION OF MOUSE SPERMATOZOA FROM INBRED AND F1-HYBRID STRAINS
CRYOPRESERVATION OF MOUSE SPERMATOZOA FROM INBRED AND F1-HYBRID STRAINS
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DOI：
10.1538/expanim1978.42.3_317
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发表时间：
1993-07-01
影响因子：
2.4
通讯作者：
TAKESHIMA, T
中科院分区：
文献类型：
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作者：
NAKAGATA, N;TAKESHIMA, T
将来自近交系（BALB/c、C3 H/He、C57 BL/6 N、CBA/JN和DBA/2N）和F1杂交系（B6 C3 F1、BDF 1和CDF 1）的小鼠附睾精子悬浮于冷冻保存溶液（18%棉子糖和3%脱脂乳的蒸馏水溶液）中，冷冻并储存在-196 ℃。在室温下解冻后，检查精子活力和可复性。所有品系的精子均成功冷冻，但冻融精子的活力和受精率均低于对照组。（Jcl：ICR品系新鲜卵母细胞经冻融精子授精后发育为原核卵母细胞和2-细胞期胚胎的比例）各品系间存在差异（运动性：C57 BL/6 N为23%，DBA/2N为62%;受精率：C57 BL/6 N为26%，DBA/2N为89%）。几乎所有冷冻精子受精的2-细胞胚胎都被移植到假孕受体的输卵管中，35-62%的2-细胞胚胎发育成正常的幼仔。
Mouse epididymal spermatozoa from inbred(BALB/c, C3H/He, C57BL/6N, CBA/JN and DBA/2N) and F1 hybrid (B6C3F1, BDF1 and CDF1) strains suspended in cryopreservation solution (18% raffinose and 3% skim milk in distilled water) were frozen and stored at -196-degrees-C . After thawing at room temperature, sperm motility and fertilizing ability were examined. Spermatozoa from all of the strains were successfully frozen, although the motility and the fertilization rates of frozen-thawed spermatozoa (the proportions of the fresh oocytes from Jcl : ICR strain which developed to pronuclear oocytes and 2-cell embryos after insemination by frozen-thawed spermatozoa) varied among strains (motility : 23% for C57BL/6N to 62% for DBA/2N ; fertilization rates : 26% for C57BL/6N to 89% for DBA/2N). Nearly all 2-cell embryos fertilized by frozen-thawed spermatozoa were transferred to the oviducts of pseudopregnant recepients and 35-62% of 2-cell embryos developed into normal young.