Zika virus infects human testicular tissue and germ cells
Zika virus infects human testicular tissue and germ cells
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DOI：
10.1172/jci121735
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发表时间：
2018-10-01
影响因子：
15.9
通讯作者：
Dejucq-Rainsford, Nathalie
中科院分区：
文献类型：
--
作者：
Matusali, Giulia;Houzet, Laurent;Dejucq-Rainsford, Nathalie
寨卡病毒（ZIKV）是一种由蚊子传播的黄病毒，可通过性行为从男性传播给女性。精液中高病毒载量和延长的病毒脱落的发现表明ZIKV在人类男性生殖道内复制，但其靶器官未知。使用器官型培养物的离体感染，我们在这里证明了ZIKV在人睾丸组织中复制并感染广泛的细胞类型，包括生殖细胞，我们也在来自ZIKV感染的供体的精液中鉴定为感染。ZIKV对人睾丸外植体的形态和激素产生没有主要的有害影响。感染诱导广泛的抗病毒反应，但没有IFN上调和最小的促炎反应，在睾丸外植体，没有细胞病变的影响。最后，我们研究了小鼠睾丸中的ZIKV感染，并将其与人类感染进行了比较。这项研究提供了关于ZIKV如何在精液中持续存在并改变精液参数的关键见解，以及测试抗病毒药物的有价值工具。
Zika virus (ZIKV) is a teratogenic mosquito-borne flavivirus that can be sexually transmitted from man to woman. The finding of high viral loads and prolonged viral shedding in semen suggests that ZIKV replicates within the human male genital tract, but its target organs are unknown. Using ex vivo infection of organotypic cultures, we demonstrated here that ZIKV replicates in human testicular tissue and infects a broad range of cell types, including germ cells, which we also identified as infected in semen from ZIKV-infected donors. ZIKV had no major deleterious effect on the morphology and hormonal production of the human testis explants. Infection induced a broad antiviral response but no IFN upregulation and minimal proinflammatory response in testis explants, with no cytopathic effect. Finally, we studied ZIKV infection in mouse testis and compared it to human infection. This study provides key insights into how ZIKV may persist in semen and alter semen parameters, as well as a valuable tool for testing antiviral agents.