Differential subcellular localization of Ror tyrosine kinase receptors in cultured astrocytes.
Differential subcellular localization of Ror tyrosine kinase receptors in cultured astrocytes.
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培养的星形胶质细胞中 Ror 酪氨酸激酶受体的差异亚细胞定位。
DOI：
10.1002/glia.20023
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Ferreira,Adriana
中科院分区：
文献类型：
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作者：
Paganoni,Sabrina;Anderson,KelsiL;Ferreira,Adriana
Ror1和Ror2属于酪氨酸激酶受体家族，在物种中高度保守。它们在整个生物体中表达，包括中枢神经系统。在本研究中，我们通过逆转录聚合酶链反应、Western blot分析和免疫细胞化学分析了Ror1和Ror2在星形胶质细胞中的表达和亚细胞定位。我们的结果表明，Ror1和Ror2在培养的星形胶质细胞中很容易检测到。他们还发现Ror1和Ror2与细胞骨架的不同组成部分有关。Ror1与F -肌动蛋白沿应力纤维共定位，Ror2与微管部分共定位。此外，我们的研究结果表明，Ror1和Ror2在培养的星形胶质细胞中经历了不同的翻译后修饰。Ror1在这些细胞中高度糖基化。相反，在Ror2中未检测到糖基化。综上所述，这些结果表明这些酪氨酸激酶受体在星形胶质细胞中的不同作用。©2004 Wiley‐Liss, Inc。
Ror1 and Ror2 belong to a family of tyrosine kinase receptors that are highly conserved among species. They are expressed throughout the organism, including the central nervous system. In the present study, we analyzed the expression and subcellular localization of Ror1 and Ror2 in astrocytes by means of reverse transcription‐polymerase chain reaction, Western blot analysis, and immunocytochemistry. Our results indicated that both Ror1 and Ror2 are readily detectable in cultured astrocytes. They also showed that Ror1 and Ror2 are associated with different components of the cytoskeleton. While Ror1 co‐localized with F‐actin along stress fibers, Ror2 partially co‐localized with microtubules. In addition, our results suggest that Ror1 and Ror2 undergo different posttranslational modifications in cultured astrocytes. Ror1 is highly glycosylated in these cells. In contrast, no glycosylation was detected in Ror2. Taken together, these results suggest distinct roles for these tyrosine kinase receptors in astrocytes. © 2004 Wiley‐Liss, Inc.