Responses to predation pressure involve similar sets of genes in two divergent species of Daphnia
Responses to predation pressure involve similar sets of genes in two divergent species of Daphnia
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DOI：
10.1111/1365-2656.13969
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发表时间：
2023-06-19
影响因子：
4.8
通讯作者：
Yin，Mingbo
中科院分区：
文献类型：
--
作者：
Zhang，Xiuping;Wolinska，Justyna;Yin，Mingbo
亲缘关系不密切的物种可以表达类似的诱导特征，但支持观察到的反应的分子机制通常是未知的，也不知道这些机制是否在这些物种之间共享。在这里，我们比较了两个物种(D.mitsukuri和D.中华按蚊)来自不同的亚属，处于幼虫和成虫发育阶段。这两个物种都受到相同的捕食威胁(鱼类凯洛蒙)，并且都表现出相似的诱导形态变化(体长缩短)。在发育的早期阶段，对捕食风险的反应导致两个物种中23个同源基因的表达水平发生了相似的变化。这些同源基因涉及107个围棋类别，与未暴露的对照相比，两个物种的同源基因在同一方向上发生了变化(过度表达或表达不足)。其中几个同源基因与DNA复制、角质层结构成分或先天免疫反应有关。在这两个物种中，差异表达(DE)基因的平均ω(dN/ds)值高于非DE基因，这表明这些基因经历了比非DE基因更大的正选择或更低的净化选择。
Species that are not closely related can express similar inducible traits, but molecular mechanisms underlying the observed responses are often unknown, nor is it known if these mechanisms are shared between such species.Here, we compared transcriptional profiles of twoDaphniaspecies (D. mitsukuriandD. sinensis) from different subgenera, at both juvenile and adult developmental stages. Both species were exposed to the same predation threat (fish kairomones), and both showed similar induced morphological changes (reduced body length).At the early developmental stage, response to predation risk resulted in similar changes in expression levels of 23 orthologues in both species. These orthologues, involved in 107 GO categories, changed in the same direction in both species (over‐ or underexpressed), in comparison to non‐exposed controls. Several of these orthologues were associated with DNA replication, structural constituents of cuticle or innate immune response. In both species, the differentially expressed (DE) genes on average had higher ω (dN/dS) values than non‐DE genes, suggesting that these genes had experienced greater positive selection or lower purifying selection than non‐DE genes.Overall, our results suggest that similar suites of genes, responding in similar ways to predation pressure, have been retained inDaphniafor many millions of years.