An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells
An in vitro study of mucoadhesion and biocompatibility of polymer coated liposomes on HT29-MTX mucus-producing cells
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DOI：
10.1016/j.ijpharm.2015.12.030
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发表时间：
2016-02-10
影响因子：
5.8
通讯作者：
Hiorth, Marianne
中科院分区：
文献类型：
--
作者：
Adamczak, Malgorzata I.;Hagesaether, Ellen;Hiorth, Marianne
由于制剂在作用部位的停留时间短，给药到口腔是一个重大的挑战。从这个角度来看，具有粘附口腔黏膜能力的纳米颗粒给药系统是有利的，因为它们可以增加治疗的有效性。带正电荷、负电荷和中性电荷的脂质体被四种不同类型的聚合物包裹：海藻酸盐、低酯果胶、壳聚糖和疏水改性乙基羟乙基纤维素。使用一种新的体外方法研究黏液粘附，该方法允许脂质体与产生黏液的融合HT29-MTX细胞系相互作用，而无需施加任何外力。在同一细胞系上进行MTT活力和细胞旁通透性试验。海藻酸盐包覆脂质体实现了高特异性（真正的）粘蛋白相互作用，具有低的细胞刺激潜能。带正电的未包被脂质体获得了最高的初始黏附，但也显示出更高的细胞刺激可能性。壳聚糖包被脂质体显示出持久黏附的最高潜力，但缺点是一般黏附（粘性）更高，刺激细胞的潜力更高。(C) 2015 Elsevier B.V.版权所有
Drug delivery to the oral cavity poses a significant challenge due to the short residence time of the formulations at the site of action. From this point of view, nanoparticulate drug delivery systems with ability to adhere to the oral mucosa are advantageous as they could increase the effectiveness of the therapy. Positively, negatively and neutrally charged liposomes were coated with four different types of polymers: alginate, low-ester pectin, chitosan and hydrophobically modified ethyl hydroxyethyl cellulose. The mucoadhesion was studied using a novel in vitro method allowing the liposomes to interact with a mucus-producing confluent HT29-MTX cell-line without applying any external force. MTT viability and paracellular permeability tests were conducted on the same cell-line. The alginate-coated liposomes achieved a high specific (genuine) mucin interaction, with a low potential of cell-irritation. The positively charged uncoated liposomes achieved the highest initial mucoadhesion, but also displayed a higher probability of cell-irritation. The chitosan-coated liposomes displayed the highest potential for long lasting mucoadhesion, but with the drawback of a higher general adhesion (tack) and a higher potential for irritating the cells. (C) 2015 Elsevier B.V. All rights reserved.