Expression of Fos-related antigens in the nucleus accumbens and associated regions following exposure to a cocaine-paired environment
Expression of Fos-related antigens in the nucleus accumbens and associated regions following exposure to a cocaine-paired environment
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DOI：
10.1046/j.1460-9568.2000.00071.x
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发表时间：
2000-06-01
影响因子：
3.4
通讯作者：
Druhan, JP
中科院分区：
文献类型：
--
作者：
Franklin, TR;Druhan, JP
本研究探讨是否条件性多动症可卡因配对环境中测量与增加表达的Fos相关抗原（FRA）内的丘脑核（NAc）和相关的前脑区域的大鼠。对三组大鼠重复注射在测试环境中的可卡因和在殖民地室中的盐水（配对组）、在测试环境中的盐水和在殖民地室中的可卡因（未配对组）或在两种环境中的盐水（对照组）。随后对所有大鼠在测试环境中进行无药物条件下的条件性多动症测试，并将其大脑取出，以便进行FRA免疫组织化学。配对组大鼠在条件反射测试中表现出条件反射性多动，并且这种行为反应与相对于未配对组和对照组的尾侧NAc、内侧前额叶皮质和外侧隔内的FRA表达增加相关。与对照组相比，配对大鼠的眶前额叶皮质、屏状核、杏仁核尾侧（基底外侧和中央区域）、丘脑室旁核、海马下托和外侧缰内的FRA表达也增加。然而，在这些后者网站的FRA水平没有显着增加相对于未配对大鼠，表明在这些地区的基因组反应并不完全依赖于上下文。条件性多动和增强FRA的表达在尾侧NAc，内侧前额叶皮层和外侧隔之间的对应关系表明，这些地区可能参与可卡因相关刺激的条件反应的表达。
This study examined whether conditioned hyperactivity measured in a cocaine-paired environment was associated with increased expression of Fos-related antigens (FRA) within the nucleus accumbens (NAc) and associated forebrain regions of rats. Three groups of rats were given repeated injections of either cocaine in the test environment and saline in the colony room (group Paired), saline in the test environment and cocaine in the colony room (group Unpaired), or saline in both environments (group Control). All rats were subsequently given a drug-free test for conditioned hyperactivity in the test environment, and their brains were removed so that FRA immunohistochemistry could be conducted. Rats in the Paired group showed conditioned hyperactivity during the conditioning test, and this behavioural response was associated with increased FRA expression within the caudal NAc, the medial prefrontal cortex and the lateral septum relative to the Unpaired and Control groups. Paired rats also showed increased FRA expression within the orbital prefrontal cortex, the claustrum, the caudal amygdala (basolateral and central regions), the paraventricular thalamic nucleus, the subiculum of the hippocampus, and the lateral habenula relative to the Control group. However, the FRA levels in these latter sites were not significantly increased relative to those of Unpaired rats, indicating that genomic responses in these regions were not entirely context dependent. The correspondence between conditioned hyperactivity and enhanced FRA expression within the caudal NAc, the medial prefrontal cortex and lateral septum suggests that these regions may participate in the expression of conditioned responses to cocaine-related stimuli.