Detection of microRNAs in prostate cancer cells by microRNA array.
Detection of microRNAs in prostate cancer cells by microRNA array.
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DOI：
10.1007/978-1-61779-083-6_6
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发表时间：
2011
影响因子：
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通讯作者：
Xiaoqing Tang;Xiaohu Tang;J. Gal;N. Kyprianou;Haining Zhu;G. Tang
中科院分区：
文献类型：
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作者：
Xiaoqing Tang;Xiaohu Tang;J. Gal;N. Kyprianou;Haining Zhu;G. Tang
MicroRNAs（miRNAs）是一类新型的非编码小RNA，在转录后水平调控基因表达，在许多重要的生物学过程和病理学发展中起着关键作用。在过去的几年中，miRNAs被认为在癌症的发生和发展中发挥重要作用。在这一章中，我们描述了一个协议，在前列腺癌细胞中的miRNA的分析和表征使用一个简单但有效的阵列平台。该阵列由553个非冗余的miRNA组成，涵盖了人类和小鼠中已知的全部miRNA。作为一个例子，在四个前列腺癌细胞系中的miRNA的谱显示，一组miRNA在雄激素依赖性和雄激素非依赖性转移性前列腺癌细胞之间差异表达。其中，通过北方印迹分析进一步验证了miR-205和miR-200 c在这两种前列腺癌细胞中的差异表达。这种全面且易于遵循的方案将有助于研究各种癌症中的miRNA，并且可以很容易地适用于各种人类疾病中的miRNA分析。
MicroRNAs (miRNAs) are a novel class of small noncoding RNAs that regulate gene expression at the posttranscriptional level and play a critical role in many important biological processes and pathological development. In the past few years, miRNAs have been implicated to play an important role in cancer initiation and development. In this chapter, we describe a protocol for the analysis and characterization of miRNAs in prostate cancer cells using a simple but effective array platform. The array is composed of 553 nonredundant miRNAs encompassing the entire set of known miRNAs in humans and mice. As an example, profiling of miRNAs in four prostate cancer cell lines has revealed that a set of miRNAs were differentially expressed between androgen-dependent and androgen-independent metastatic prostate cancer cells. Among them, the differential expression of miR-205 and miR-200c were further validated by Northern blot analysis in these two types of prostate cancer cells. This comprehensive and easy-to-follow protocol will be useful for studying miRNAs in various cancers and can be readily adapted for miRNA analysis in a variety of human diseases.