IDENTIFICATION AND MAPPING OF THE TRANSCRIPTIONAL AND TRANSLATIONAL PRODUCTS OF THE YEAST PLASMID, 2 MU-CIRCLE
IDENTIFICATION AND MAPPING OF THE TRANSCRIPTIONAL AND TRANSLATIONAL PRODUCTS OF THE YEAST PLASMID, 2 MU-CIRCLE
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DOI：
10.1016/0092-8674(79)90098-9
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发表时间：
1979-01-01
期刊：
影响因子：
64.5
通讯作者：
CHOW, L
中科院分区：
文献类型：
--
作者：
BROACH, JR;ATKINS, JF;CHOW, L
Two major and approximately 10 minor poly(A)-containing RNA species in Saccharomyces cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2.mu. circle were identified. The 2 major species, which are 1325 and 1275 bases in length, are transcribed from the 2 unique halves of the plasmid and extend into the inverted repeat sequences which separate the unique regions. The map positions of the minor transcripts, which are 350-2600 bases in length, indicate that except for a small region of the genome in which no transcription is observed, both strands of the entire 2.mu. circle genome are transcribed. RNA transcribed from 2.mu. circular DNA is used to program the synthesis of specific proteins in yeast: that is, yeast RNA complementary to 2.mu. circle DNA can be translated in vitro to produce specific polypeptides of substantial size. Finally, the pattern of transcription of 2.mu. circle suggests the possibility that messenger RNA species are derived by cleavage of larger transcripts, and in addition, that the intramolecular recombination of 2.mu. circle which occurs in yeast functions as a genetic switch to allow separate expression of 2 sets of genes on the 2.mu. circle genome.
