Presence of functional dendritic cells in patients chronically infected with hepatitis C virus
Presence of functional dendritic cells in patients chronically infected with hepatitis C virus
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DOI：
10.1182/blood-2003-04-1339
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发表时间：
2004-02-01
期刊：
影响因子：
20.3
通讯作者：
Rice, CM
中科院分区：
文献类型：
--
作者：
Longman, RS;Talal, AH;Rice, CM
慢性丙型肝炎病毒（HCV）感染患者中缺乏大量的HCV反应性CD8（+）T淋巴细胞（CTL），这导致了对该人群中树突状细胞（DC）功能的研究，认为这是该缺陷的潜在原因。一些研究已经显示慢性感染患者中单核细胞衍生的DC受损的证据。由于这些数据很难与慢性HCV患者具有免疫能力的事实相一致，我们重新评估了这一发现，与未感染的对照组相比，仔细评估了患者DC的表型标记物和功能活性。与这些先前的研究相反，13例慢性HCV患者中有13例的DC表达典型的成熟标志物。这些成熟DC能够引发同种异体T淋巴细胞，以及刺激流感特异性记忆T细胞。这一发现与临床和免疫学数据一致，即患者免疫库中的缺陷是HCV特异性的，并表明需要改进模型以了解DC在HCV发病机制中的作用。
The absence of expanded numbers of hepatitis C virus (HCV)-reactive CD8(+) T lymphocytes (CTLs) in patients chronically infected with HCV has led to the investigation of dendritic cell (DC) function in this population as a potential cause for this defect. Several studies have shown evidence for impaired monocyte-derived DCs in chronically infected patients. As it is difficult to reconcile these data with the fact that patients with chronic HCV are immune competent, we re-evaluated this finding, carefully assessing phenotypic markers and functional activity of patient DCs as compared with noninfected controls. In contrast to these prior studies, DCs from 13 of 13 chronic HCV patients expressed typical maturation markers. These mature DCs were capable of priming allogeneic T lymphocytes, as well as stimulating influenza-specific memory T cells. This finding is consistent with clinical and immunologic data that the deficit in the patient's immune repertoire is HCV-specific and suggests that refined models are required for understanding the role of DCs in HCV pathogenesis.