Fabrication of 3D micropatterns with high hosting cell efficiency and their effects on osteogenic differentiation of MSCs
Fabrication of 3D micropatterns with high hosting cell efficiency and their effects on osteogenic differentiation of MSCs
复制标题
具有高宿主细胞效率的 3D 微图案的制作及其对 MSC 成骨分化的影响
DOI：
10.1016/j.matlet.2021.130045
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发表时间：
2021
影响因子：
3
通讯作者：
Zhang Shengmin
中科院分区：
文献类型：
--
作者：
Wu Feige;Yang Gaojie;Li Yan;Wang Yifan;Tan Shenglong;Du Yingying;Zhong Zhenyu;Lan Ziyang;Zhang Shengmin
三维微图案是研究材料与细胞相互作用的合适模板，但挑战之一是宿主细胞效率低。因此，通过一种新技术制造了矩形、三角形、圆形和正方形的3D微图案，该技术将干细胞的生长限制在3D微图案内，并实现了超过90%的宿主细胞效率。体外免疫荧光和体内组织化学检测结果显示，矩形和三角形三维微图案组的成骨相关蛋白和endotoxin雅普/TAZ的表达高于圆形和正方形组，阐明了三维微图案通过力学信号雅普/TAZ调控干细胞分化的分子机制。总之，我们的高宿主细胞效率的3D微图案通过图案诱导的机械刺激调节干细胞分化，影响骨组织的再生。该项目将为开发基于表面微纳工程的下一代3D图案诱导组织工程材料提供可行的范例。
3D micropatterns were suitable template for studying the interactions between materials and cells, but one of the challenges was the low hosting cell efficiency. Thus, 3D micropatterns of the rectangle, triangle, circle and square were fabricated by a novel technology, which limited the growth of stem cells within the 3D micropatterns and achieved a hosting cell efficiency of more than 90%. Further, results of immunofluorescencein vitroand immunohistochemistryin vivo, showed that the expression of osteogenic associated proteins and endonuclear YAP/TAZ of rectangle and triangle 3D micro-patterns groups were higher than circle and square groups, elucidating the molecular mechanism of 3D micro-patterns regulating stem cell differentiation through mechanical signal YAP/TAZ. In conclusion, our 3D micro-patterns of high hosting cell efficiency regulated stem cell differentiation by a pattern-induced mechanical stimulus, affecting the regeneration of bone tissue. This project will provide a feasible paradigm for developing the next generation of 3D-patterns induced tissue engineering materials based on surface micro-nano engineering.