FOCAL CEREBRAL-ISCHEMIA IN THE RAT .1. DESCRIPTION OF TECHNIQUE AND EARLY NEUROPATHOLOGICAL CONSEQUENCES FOLLOWING MIDDLE CEREBRAL-ARTERY OCCLUSION
FOCAL CEREBRAL-ISCHEMIA IN THE RAT .1. DESCRIPTION OF TECHNIQUE AND EARLY NEUROPATHOLOGICAL CONSEQUENCES FOLLOWING MIDDLE CEREBRAL-ARTERY OCCLUSION
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DOI：
10.1038/jcbfm.1981.6
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发表时间：
1981-01-01
影响因子：
6.3
通讯作者：
TEASDALE, GM
中科院分区：
文献类型：
--
作者：
TAMURA, A;GRAHAM, DI;TEASDALE, GM
本文描述了一种闭塞大鼠大脑中近动脉干的方法。手术是在麻醉下通过一个小的颞下颅骨切除术进行的。闭塞后，3只动物灌注炭黑，8只动物灌注FAM固定剂（40%甲醛、冰醋酸和甲醇）。研究结果与假手术动物进行了比较。炭黑研究表明，每只动物的灌注受损区域与闭塞动脉的区域相对应。神经病理学研究无一例外地显示在皮层和基底节区存在缺血性脑损伤。每只动物的额叶皮质都参与其中，新纹状体的外侧部分也是如此；大多数动物的感觉运动皮层和听觉皮层受累，枕皮质和内侧纹状体受累较少。在假手术动物中，由缺血造成的损伤可以很容易地与手术部位的局部小病变区分开来。在啮齿动物大脑中产生一致的局灶性缺血性损伤的能力提供了一种充分可重复的技术方法，可以使用最近开发的放射自显影和神经化学方法来研究缺血的病理生理学。
A procedure for occluding the stem of the proximal middle cerebral artery of the rat is described. The operation is performed under anesthesia through a small subtemporal craniectomy. After occlusion, 3 animals were perfused with carbon black and 8 with a FAM fixative (40% formaldehyde, glacial acetic acid and methanol). The findings were compared with sham-operated animals. Carbon black studies demonstrated an area of impaired perfusion corresponding to the territory of the occluded artery in each animal. Neuropathological studies invariably showed that there was ischemic brain damage in the cortex and basal ganglia. The frontal cortex was involved in every animal, as was the lateral part of the neostriatum; the sensorimotor and auditory cortex were involved in most animals, while the occipital cortex and medial striatum were involved only infrequently. The damage produced by ischemia could be readily distinguished from the small local lesion seen at the surgical site in sham-operated animals. The ability to produce a consistent focal ischemic lesion in the rodent brain provides a technical approach that is sufficiently reproducible to enable investigation of the pathophysiology of ischemia using recently developed autoradiographic and neurochemical methods.