The potato tuber transcriptome: analysis of 6077 expressed sequence tags
The potato tuber transcriptome: analysis of 6077 expressed sequence tags
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DOI：
10.1016/s0014-5793(01)02888-5
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发表时间：
2001-10-05
期刊：
影响因子：
3.5
通讯作者：
Nielsen, KL
中科院分区：
文献类型：
--
作者：
Crookshanks, M;Emmersen, J;Nielsen, KL
这是利用表达序列标签测序研究块茎贮藏器官生物合成潜力的首次报道。以马铃薯(Solanum tuberosum var.)成熟块茎为材料，构建了马铃薯成熟块茎的cDNA文库。Kuras)。从6077个克隆中获得的部分序列被组装成828个簇和1533个单态。平均阅读长度为592bp，其中2254个克隆为全长。5717个克隆与其他生物的基因具有同源性。与蛋白质合成、蛋白质去向和细胞防御有关的基因在块茎中占优势，而在匍匐茎、地上部和叶中相对较少。1063个克隆为块茎所特有。淀粉代谢酶在块茎和匍匐茎中的转录本的相对水平相似。(C)2001年欧洲生化学会联合会。爱思唯尔科学公司出版。版权所有。
This is the first report of the biosynthetic potential of a tuber storage organ investigated by expressed sequence tag sequencing. A cDNA library was generated from the mature tuber of field grown potato (Solanum tuberosum var. Kuras). Partial sequences obtained from 6077 clones were assembled into 828 clusters and 1533 singletons. The average read length was 592 bp, and 2254 clones were full length. 5717 clones showed homology to genes from other organisms. Genes involved in protein synthesis, protein destination and cell defense predominated in tuber compared to stolon, shoot and leaf organs. 1063 clones were unique to tuber. Transcripts of starch metabolizing enzymes showed similar relative levels in tuber and stolon. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.