In Vivo Assessment of Rodent Retinal Structure Using Spectral Domain Optical Coherence Tomography
In Vivo Assessment of Rodent Retinal Structure Using Spectral Domain Optical Coherence Tomography
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DOI：
10.1007/978-1-4614-0631-0_61
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发表时间：
2012-01-01
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影响因子：
--
通讯作者：
Seeliger, Mathias W.
中科院分区：
文献类型：
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作者：
Fischer, M. Dominik;Huber, Gesine;Seeliger, Mathias W.
为了确定市售光学相干断层扫描（OCT）设备（Spectralis™ HRA + OCT，Heidelberg Engineering）用于小动物视网膜成像的潜力，我们实现了对该第三代OCT系统进行调整，以获得和量化具有获得性和遗传性视网膜变性的模型中啮齿动物视网膜的高分辨率形态切片。遗传诱导（rd 1、rho−/−、RPE 65）和获得性视网膜变性（光损伤）与组织学结果相似，并且可以按照时间轴进行随访。我们能够在视觉研究中使用的各种已建立的动物模型中检测和分析广泛的视网膜病变。由于这种技术允许纵向研究设计，它将有助于表征疾病动力学，同时减少所需的研究动物数量。在动物和临床研究中使用相同的结局指标，甚至相同的诊断设备，有可能简化转化方法，例如，评估假定的治疗干预措施。
To determine the potential of a commercially available optical coherence tomography (OCT) device (Spectralis™ HRA + OCT, Heidelberg Engineering) for small animal retinal imaging, we achieved to adapt this third generation OCT system to obtain and quantify high-resolution morphological sections of rodent retina in models with acquired and inherited retinal degenerations. Genetically induced (rd1, rho−/−, RPE65) and acquired retinal degeneration (light damage) was similarly clear as in histology and could be followed in a timeline fashion. We were able to detect and analyze a wide range of retinal pathology in a variety of established animal models used in vision research. As this technique allows longitudinal study designs, it will facilitate characterization of disease dynamics while reducing the numbers of study animals needed. Use of identical outcome measures and even the same diagnostic device in animal and clinical studies bears the potential to streamline translational approaches, e.g., in the assessment of putative therapeutic interventions.