Development of multifunctional hyaluronan-coated nanoparticles for imaging and drug delivery to cancer cells.
Development of multifunctional hyaluronan-coated nanoparticles for imaging and drug delivery to cancer cells.
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DOI：
10.1021/bm300046h
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发表时间：
2012-04-09
影响因子：
6.2
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
El-Dakdouki MH;Zhu DC;El-Boubbou K;Kamat M;Chen J;Li W;Huang X
目前，开发用于癌症监测和化疗的多功能治疗平台具有很高的兴趣。在这里，我们报道透明质酸（HA）涂层的超顺磁性氧化铁纳米颗粒（HA- spion）是一种很有前途的靶向成像和药物递送系统。与癌细胞孵育后，HA- spion被癌细胞迅速吸收，并且与未包被HA的NPs相比，HA- spion被癌细胞内化的程度要高得多。HA-SPION的高磁弛豫加上增强的摄取使癌细胞的磁共振成像成为可能。此外，阿霉素（DOX）通过酸反应连接剂附着在纳米颗粒上。虽然HA-SPION对细胞没有毒性，但DOX-HA-SPION对药物敏感和多重耐药的癌细胞的杀伤能力比游离DOX强得多。这归因于癌细胞中游离DOX和DOX- ha - spion的不同摄取机制和细胞分布。
Currently, there is high interest in developing multifunctional theranostic platforms for cancer monitoring and chemotherapy. Herein, we report hyaluronan (HA)-coated superparamagnetic iron oxide nanoparticles (HA-SPION) as a promising system for targeted imaging and drug delivery. When incubated with cancer cells, HA-SPIONs were rapidly taken up and the internalization of HA-SPION by cancer cells was much higher than the NPs without HA coating. The high magnetic relaxivity of HA-SPION coupled with enhanced uptake enabled magnetic resonance imaging of cancer cells. Furthermore, doxorubicin (DOX) was attached onto the nanoparticles through an acid responsive linker. While HA-SPION was not toxic to cells, DOX-HA-SPION was much more potent than free DOX to kill not only drug-sensitive but also multi-drug-resistant cancer cells. This was attributed to differential uptake mechanisms and cellular distributions of free DOX and DOX-HA-SPION in cancer cells.