Correlation of two bioadhesion assays: the everted sac technique and the CAHN microbalance.
Correlation of two bioadhesion assays: the everted sac technique and the CAHN microbalance.
复制标题
两种生物粘附测定的相关性：外翻囊技术和 CAHN 微量天平。
DOI：
10.1016/s0168-3659(99)00109-1
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Mathiowitz,E
中科院分区：
文献类型：
--
作者：
Santos,CA;Jacob,JS;Hertzog,BA;Freedman,BD;Press,DL;Harnpicharnchai,P;Mathiowitz,E
这一贡献与用于确定生物粘附的两种体外方法相关。一种方法是外翻肠囊技术，是一种生物粘附的被动测试，涉及多个聚合物微球和一段外翻肠组织。另一种方法是 CAHN 微天平，采用 CAHN 动态接触角分析仪和改进的软件来记录从肠组织中拉出单个聚合物微球时测量到的张力。这项研究表明，当用于量化聚合物微球系统的生物粘附性质时，CAHN 和外翻囊实验会产生类似的结果。在一种方法中表现出高粘附力的聚合物在另一种方法中也表现出高生物粘附力；使用 CAHN 微天平测量具有高断裂强度和拉伸功的聚合物，也可以使用翻转囊法获得高结合百分比。这里测试和报道的聚合物是聚己内酯和不同比例的聚富马酸-癸二酸酐共聚物。这种相关性的结果表明，每种方法单独都是生物粘附的有价值的指标。
This contribution correlates two in vitro methods utilized to determine bioadhesion. One method, the everted intestinal sac technique, is a passive test for bioadhesion involving several polymer microspheres and a section of everted intestinal tissue. The other method, the CAHN microbalance, employs a CAHN dynamic contact angle analyzer with modified software to record the tensile forces measured as a single polymer microsphere is pulled from intestinal tissue. This study demonstrates that CAHN and everted sac experiments yield similar results when used to quantify the bioadhesive nature of polymer microsphere systems. A polymer showing high adhesion in one method also demonstrates high bioadhesion in the other method; polymers that exhibit high fracture strength and tensile work measurements with the CAHN microbalance also yield high binding percentages with the everted sac method. The polymers tested and reported here are poly(caprolactone) and different copolymer ratios of poly(fumaric-co-sebacic anhydride). The results of this correlation demonstrate that each method alone is a valuable indicator of bioadhesion.