Measuring D-serine efflux from mouse cortical brain slices using online microdialysis-capillary electrophoresis.
Measuring D-serine efflux from mouse cortical brain slices using online microdialysis-capillary electrophoresis.
复制标题
使用在线微透析毛细管电泳测量小鼠皮质脑切片的 D-丝氨酸流出。
DOI：
10.1002/elps.200500770
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Bowser,MichaelT
中科院分区：
文献类型：
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作者：
O'Brien,KylieB;Bowser,MichaelT
Efflux of a number of important neurochemicals, includingD‐serine,L‐serine, taurine, glutamate, and γ‐aminobutyric acid (GABA), from mouse cortical brain slices housed in a 7 µL perfusion chamber was monitored using online microdialysis‐CE (MD‐CE). Analyte concentrations could be measured every 20–27 s using the MD‐CE instrument. Stimulation with high potassium induced increased release ofD‐serine. Kainic acid (KA) inducedD‐serine release, but this release was not blocked by 6‐cyano‐7‐nitroquinoxaline‐2,3‐dione, suggesting that α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole‐propionic acid/KA receptors do not mediateD‐serine release. Application ofL‐serine, the precursor ofD‐serine, resulted in increased extracellularD‐serine concentrations.L‐Cysteine also increased extracellularD‐serine levels in a partially Na+‐dependent manner. The observed effects upon application ofL‐serine andL‐cysteine support the involvement of ASC neutral amino acid transporters in regulating the extracellular concentration ofD‐serine concentration through competitive inhibition of uptake or increased release through heteroexchange.
