Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression
Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression
复制标题
电针通过调节抑郁症大鼠模型中的 5-HT 受体恢复海马突触可塑性
DOI：
10.1016/j.brainresbull.2018.03.004
复制
发表时间：
2018-05-01
影响因子：
3.8
通讯作者：
Xu, Shifen
中科院分区：
文献类型：
--
作者：
Han, Xuke;Wu, Huangan;Xu, Shifen
目的：观察电针对Wistar京都(WKY)抑郁模型大鼠的影响，探讨电针对大鼠海马CA1区神经元突触可塑性的可能机制。方法：雄性WKY大鼠随机分为3组(电针组、假电针组和模型组，n=8)，Wistar大鼠作为正常对照组(n=8)。针刺百会、阴塘穴，每日1次，疗程3周。假电针组在无电刺激的情况下，将针刺针刺至穴位表面。第21天进行强迫游泳试验(FST)、旷场试验(OFT)和蔗糖嗜好试验(SPT)。行为学测试结束后，电生理记录大鼠海马脑片Schaffer侧支CA1区突触长时程增强(LTP)，Western blotting检测海马CA1区5-HTT、5-HT1a和5-HT1B蛋白水平。结果与Sham电针组和模型组比较，电针组大鼠FST内不动次数明显减少，蔗糖摄入量明显增加。电针组大鼠OFT的中枢时间和总运动时间较模型组显著延长。与假电针组和模型组比较，电针组的fEPSP斜率显著增加，LTP诱导成功。与假电针组和模型组比较，电针组大鼠海马CA1区5-HTT蛋白表达显著降低。结论：电针可通过恢复海马CA1区突触可塑性而改善抑郁样行为，其机制可能主要是通过调节5-羟色胺受体水平来实现的。
Objective: The study aimed to determine the effect of electroacupuncture (EA) on Wistar Kyoto (WKY) depressive model rats and explore the possible mechanism of EA on hippocampal CA1 region neuronal synaptic plasticity.Methods: The male WKY rats were randomized to three experimental groups (EA, Sham EA, and Model group, n = 8/group), and Wistar rats as the normal control group (n = 8). EA treatment was administered once daily for 3 weeks at acupuncture points Baihui (GV20) and Yintang (EX-HN3). In the Sham EA group, acupuncture needles were inserted superficially into the acupoints without electrical stimulation. On day 21, the forced swimming test (FST), open field test (OFT) and sucrose preference test (SPT) were conducted. After the behavioral tests, long-term potentiation (LTP) was evoked at Schaffer collateral-CA1 synapses in hippocampal slices in vitro by electrophysiological recording, 5-HTT, 5-HT1A and 5-HT1 B protein levels in the hippocampus CA1 region were examined by using Western blot.Result EA significantly decreased immobility in FST and improved sucrose intake compared with the Sham EA and Model groups. The center time and total move time in OFT were significantly increased in the EA group compared to the Model group. Compared with those of the Sham EA and Model groups, the fEPSP slope of the EA group increased significantly, and the LTP induction was successful. EA significantly decreased 5-HTT protein expression in the hippocampus CA1 region in comparison to the Sham EA and Model groups. Additionally, EA down regulated the 5-HT1A protein expression in the hippocampus CA1 region in comparison to the Sham EA group.Conclusion: EA could ameliorate depressive-like behaviors by restoring hippocampus CA1 synaptic plasticity, which might be mainly mediated by regulating 5-HT receptor levels.