Genetic analysis of the O-antigen gene clusters of Yersinia pseudotuberculosis O:6 and O:7
Genetic analysis of the O-antigen gene clusters of Yersinia pseudotuberculosis O:6 and O:7
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DOI：
10.1093/glycob/cwr010
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发表时间：
2011-09-01
期刊：
影响因子：
4.3
通讯作者：
Reeves, Peter R.
中科院分区：
文献类型：
--
作者：
Cunneen, Monica M.;Pacinelli, Elvia;Reeves, Peter R.
在21种基于O-多糖（OPS）O-抗原的假结核耶尔森氏菌血清型中，O：6和O：7的血清型是不寻常的，因为它们都含有粘三糖（4-酮基-3，6-双脱氧-D-甘露糖或4-酮基-3，6-双脱氧-L-木糖-己糖），这在该物种中还没有报道过，O：6 OPS还含有耶尔森氏糖A（4-C[（R）-1-羟乙基]-3，6-双脱氧-D-木糖-己糖），这是另一种不寻常的双脱氧己糖。耶氏酵母中在假结核病中，用于OPS合成的基因通常在hemH和gsk基因座之间聚集在一起。在这里，我们提出了Y的OPS基因簇序列。假结核O：6和O：7，以及合成这些OPS所需的基因的位置，除了关于一些糖基转移酶功能的分配仍然是模糊的。O：6和O：7基因簇彼此有很多共同之处，但与已经测序的13个基因簇的组有很大不同，这些基因簇具有几个特征和序列相似性。我们还提出了一个可能的事件序列的衍生O：6和O：7基因簇从最密切相关的一组13个先前测序。
Among the 21 O-polysaccharide (OPS) O-antigen-based serotypes described for Yersinia pseudotuberculosis, those of O:6 and O:7 are unusual in that both contain colitose (4-keto-3,6-dideoxy-D-mannose or 4-keto-3,6-dideoxy-L-xylo-hexose), which has not otherwise been reported for this species, and the O:6 OPS also contains yersiniose A (4-C[(R)-1-hydroxyethyl]-3,6-dideoxy-D-xylo-hexose), another unusual dideoxyhexose sugar. In Y. pseudotuberculosis, the genes for OPS synthesis generally cluster together between the hemH and gsk loci. Here, we present the sequences of the OPS gene clusters of Y. pseudotuberculosis O:6 and O:7, and the location of the genes required for synthesis of these OPSs, except that there is still ambiguity regarding allocation of some of the glycosyltransferase functions. The O:6 and O:7 gene clusters have much in common with each other, but differ substantially from the group of 13 gene clusters already sequenced, which share several features and sequence similarities. We also present a possible sequence of events for the derivation of the O:6 and O:7 gene clusters from the most closely related set of 13 sequenced previously.