EVANS BLUE FLUORESCENCE - QUANTITATIVE AND MORPHOLOGICAL EVALUATION OF VASCULAR-PERMEABILITY IN ANIMAL-TISSUES
EVANS BLUE FLUORESCENCE - QUANTITATIVE AND MORPHOLOGICAL EVALUATION OF VASCULAR-PERMEABILITY IN ANIMAL-TISSUES
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DOI：
10.1016/0165-0270(83)90050-x
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发表时间：
1983-01-01
影响因子：
3
通讯作者：
LUNDBERG, JM
中科院分区：
文献类型：
--
作者：
SARIA, A;LUNDBERG, JM
使用染料Evans蓝监测血管蛋白渗漏。荧光法测定大鼠气管组织的甲酰胺提取物中伊文思蓝的蛋白质渗漏诱导后，电迷走神经刺激，并与广泛使用的比色检测进行比较。荧光法比比色法灵敏约100倍。伊文思蓝荧光（激发波长620 nm，发射波长680 nm）用于气管组织冷冻分泌物的显微镜研究。神经电刺激后渗出的伊文思蓝主要见于气管上皮下，与组织成分有明显的结合。显然，伊文思蓝荧光可以应用于蛋白质渗漏的组织定位在微观水平上。
The dye Evans blue was used to monitor vascular protein leakage. Fluorometric measurement of Evans blue in formamide extracts of rat tracheal tissue was performed after induction of protein leakage by electrical vagus nerve stimulation and compared with the widely used colorimetric detection. The fluorescence method was approximately 100 times more sensitive than the colorimetric method. Evans blue fluorescence (excitation at 620 nm, emission at 680 nm) was used for microscopic investigation of cryostate secretions of tracheal tissue. Extravasated Evans blue after electrical nerve stimulation was mainly found in the subepithelial layer of the trachea obviously bound to tissue constituents. Apparently, Evans blue fluorescence can be applied for tissue localization of protein leakage at the microscopic level.