Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
Molecular cloning and expression patterns of the Vasa gene from Rana nigromaculata (Amphibia: Anura)
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黑斑蛙（两栖类：无尾目）Vasa 基因的分子克隆和表达模式
DOI：
10.1590/s1984-46702009000200014
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发表时间：
2009-06-01
期刊：
影响因子：
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通讯作者：
Wang, Jingjing
中科院分区：
文献类型：
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作者：
Jia, Rui;Nie, Liu-Wang;Wang, Jingjing
Vasa蛋白是atp依赖性RNA解旋酶DEAD （Asp-Glu-Alu-Asp）盒子家族的一员。Vasa基因在许多后生动物的生殖系细胞中特异表达，在配子发生和生殖调节中起关键作用。本文从蛙蛙（Rana nigromaculata Hallowell, 1861）中分离出Vasa基因的全长cDNA序列。编码398个氨基酸残基的开放阅读框（ORF）有9个保守基序。根据氨基酸序列的相似性，Vasa基因的系统发育分析与脊索动物到哺乳动物的进化关系一致。此外，利用逆转录聚合酶链式反应（RT-PCR）技术对RnVasa mRNA的表达模式分析显示，RnVasa mRNA在睾丸、卵巢和肾脏中表达水平较高，而在其他组织中几乎没有检测到信号，这表明它可能在配子发生过程中发挥作用。
The Vasa protein is a member of the DEAD (Asp-Glu-Alu-Asp) box family of ATP-dependent RNA helicases. The Vasa gene is specifically expressed in germ-line cells of many metazoans and is known to play a critical role in gametogenesis and reproductive regulation. In this paper, we isolate the full length cDNA sequence of the Vasa gene from the frog Rana nigromaculata Hallowell, 1861. The open reading frame (ORF) encoding 398 amino acid residues has nine conserved motifs. According to the similarities at the amino acid sequenceythe phylogenetic analysis of Vasa gene was consistent with the evolution relationships from chordates to mammals. Furthermore, the expression pattern analysis of RnVasa mRNA, using the technique of Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), showed a high level of transcripts in testis, ovary and kidney, whereas little to no signal was detected in other tissues, which suggests that it may play a role during gametogenesis.