Turbiscan Lab® Expert analysis of the biological demulsification of a water-in-oil emulsion by two biodemulsifiers
Turbiscan Lab® Expert analysis of the biological demulsification of a water-in-oil emulsion by two biodemulsifiers
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DOI：
10.1016/j.jhazmat.2011.03.028
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发表时间：
2011-06-15
影响因子：
13.6
通讯作者：
Deng, Hui-ping
中科院分区：
文献类型：
--
作者：
Liu, Jia;Huang, Xiang-feng;Deng, Hui-ping
研究了产碱杆菌S-XJ-1在不同培养条件下产生的两种破乳剂对油包水乳状液的长期破乳过程。以石蜡为底物，在初始培养pH为10时得到生物破乳剂I，以废煎炸油为底物，在pH为7时得到生物破乳剂II。前者具有更高的破乳能力和界面活性比后者。采用瓶试验、显微镜和Turbiscan Lab（R）Expert研究生物破乳过程。结果表明，生物破乳剂降低界面张力的能力比降低表面张力的能力在破乳过程中起着更重要的作用。由于其两亲性，破乳过程开始于生物破乳剂吸附到水-油界面上。然后，生物破乳剂与乳化剂反应，因为它们的界面活性。结果，从分散液滴的表面去除薄液膜并且发生聚结。这导致分散液滴的沉降和连续相的澄清。Turbiscan Lab（R）Expert可用于评价破乳效率和分析不同生物破乳剂的去稳定过程。该方法是从其它生物制品中筛选高效破乳剂的一种快速、准确的方法。(C)2011 Elsevier B. V.保留所有权利。
The long-term destabilization process of a water-in-oil emulsion was investigated with two different biodemulsifiers produced under different culture conditions by Alcaligenes sp. S-XJ-1. Biodemulsifier I was obtained by using paraffin as substrate at initial culture pH of 10 and biodemulsifier II was produced with waste frying oils at pH of 7. The former exhibited higher demulsifying ability and interfacial activity than the latter. Bottle test, microscopy and Turbiscan Lab (R) Expert were used to investigate the biological demulsification process. It was found that biodemulsifiers' ability to decrease the interfacial tension played a more important role in demulsification than their ability to decrease the surface tension. Owing to their amphiphilic nature, demulsification process began with the adsorption of the biodemulsifiers onto the water-oil interface. Then the biodemulsifiers reacted with the emulsifiers because of their interfacial activity. As a result, thin liquid film was removed from the surface of dispersed droplets and coalescence occurred. This led to the settling of the dispersed droplets and the clarification of the continuous phase. Turbiscan Lab (R) Expert can be used to evaluate the demulsification efficiency and to analyze the destabilization process of different biodemulsifiers. It is a rapid and accurate method to screen high-efficiency demulsifiers from other bioproducts. (C) 2011 Elsevier B.V. All rights reserved.