Developmental elimination of ectopic projection sites for the transgenic OR gene that has lost zone specificity in the olfactory epithelium
Developmental elimination of ectopic projection sites for the transgenic OR gene that has lost zone specificity in the olfactory epithelium
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DOI：
10.1046/j.1460-9568.2003.02998.x
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发表时间：
2003-11-01
影响因子：
3.4
通讯作者：
Sakano, H
中科院分区：
文献类型：
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作者：
Nakatani, H;Serizawa, S;Sakano, H
在啮齿类动物中，嗅觉受体（OR）基因在嗅上皮（OE）的四个区域之一中表达，并且表达相同OR的嗅觉感觉神经元（OSN）将其轴突投射到嗅球（OB）上的一组特定肾小球。利用酵母人工染色体（YAC）转基因系统，我们分析了小鼠OR基因MOR29 A的MOR28簇位于14号染色体上的表达。虽然内源性MOR29A的表达仅限于最背内侧区，但转基因MOR29A（Tg MOR29A）在OE的所有四个区域中表达。当分析转基因小鼠的OB时，发现表达Tg MOR29A的OSN的轴突不仅投射到OB的背侧，而且也投射到OB的腹侧。腹侧的异位投射部位在出生后发育过程中逐渐消失。鼻孔阻塞阻止这种消除，这表明气味刺激参与消除异位投射部位。
In rodents, olfactory receptor (OR) genes are expressed in one of four zones in the olfactory epithelium (OE), and olfactory sensory neurons (OSNs) expressing the same OR project their axons to a specific set of glomeruli on the olfactory bulb (OB). Using the yeast artificial chromosome (YAC) transgenic system, we have analysed the expression of the murine OR gene MOR29A of the MOR28 cluster located on chromosome 14. Although expression of the endogenous MOR29A was restricted to the most dorsomedial zone, the transgenic MOR29A (Tg MOR29A) was expressed in all four zones of the OE. When the OB of the transgenic mouse was analysed, the axons of the OSNs expressing Tg MOR29A were found to project not only to the dorsal side but also to the ventral side of the OB as well. The ectopic projection sites on the ventral side gradually disappear during postnatal development. Naris occlusion prevents this elimination, suggesting that odorant stimulation is involved in eliminating the ectopic projection sites.