Homeodomain transcription factors in the development of subsets of hindbrain reticulospinal neurons
Homeodomain transcription factors in the development of subsets of hindbrain reticulospinal neurons
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DOI：
10.1016/j.mcn.2004.06.016
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发表时间：
2005-01-01
影响因子：
3.5
通讯作者：
Varela-Echavarría, A
中科院分区：
文献类型：
--
作者：
Cepeda-Nieto, AC;Pfaff, SL;Varela-Echavarría, A
后脑网状脊髓神经元参与由脊髓成分介导的复杂神经功能，包括姿势控制和呼吸和心血管功能的调节。最近对鸡、小鼠和大鼠胚胎的描述性研究为不同网状脊髓核的发育及其轴突投射途径进入脊髓的建立提供了解剖学见解。在这项研究中，我们已经解决了这个过程的分子控制。鸡和小鼠胚胎中网状脊髓神经元的逆行标记结合同源结构域因子Lhx 1/Lhx 5，Lhx 3/Lhx 4和Chx 10的免疫染色已经定义了标记具有不同轴突投射模式的神经元子集的转录代码。使用卵内电穿孔的功能获得和功能丧失实验暗示这些转录因子在网状脊髓神经元身份的确定中。此外，我们的研究揭示了分析的转录因子之间的新基因相互作用，这可能决定网状脊髓轴突投射的最终模式。(C)2004年爱思唯尔公司All rights reserved.
Hindbrain reticulospinal neurons are involved in complex neural functions that are mediated by spinal elements, including posture control and modulation of respiration and cardiovascular function. Recent descriptive studies with chick, mouse, and rat embryos have provided anatomical insight into the development of the different reticulospinal nuclei and the establishment of their axonal projection pathways into the spinal cord. In this study, we have addressed the molecular control of this process. Retrograde labeling of reticulospinal neurons in chick and mouse embryos combined with immunostaining for the homeodomain factors Lhx1/Lhx5, Lhx3/Lhx4, and Chx10 have defined transcriptional codes that label subsets of neurons with different axon projection patterns. Gain of function and loss of function experiments using in ovo electroporation implicate these transcription factors in the determination of reticulospinal neuron identity. Furthermore, our studies reveal novel gene interactions between the transcription factors analyzed that may determine the final patterns of reticulospinal axon projection. (C) 2004 Elsevier Inc. All rights reserved.