On demand nanoliter-scale microfluidic droplet generation, injection, and mixing using a passive microfluidic device.
On demand nanoliter-scale microfluidic droplet generation, injection, and mixing using a passive microfluidic device.
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DOI：
10.1063/1.4907895
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发表时间：
2015-02
期刊：
影响因子：
3.2
通讯作者：
U. Tangen;Abhishek Sharma;P. Wagler;J. McCaskill
中科院分区：
文献类型：
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作者：
U. Tangen;Abhishek Sharma;P. Wagler;J. McCaskill
我们在这里提出并描述了一种可编程的纳米升规模的按需液滴装置，它可以单独使用，也可以很容易地集成到低成本的单层快速成型微流体系统中，用于广泛的用户应用。无源微流体装置允许外部（现成的）电子控制捏阀编程纳升级水滴从多达9个不同的输入到中央出口通道的交付。输入可以是连续的含水流体流，也可以是嵌入在载体流体中的微升级含水塞，在这种情况下，可用于实验的有效输入溶液的数量不再受到严格限制（100 s-1000 s）。用该装置进行了纳米级液滴测序输出和纳米级液滴混合。在开源微流控仿真软件和等效电路模型的支持下，通过多次硬件迭代实现了器件的几何和压力关系优化。设备内压力关系的必要模块化控制是通过流体动力屏障和三个不同通道高度的匹配阻力通道，定制并行可逆微流体I/O连接，低死体积箝位阀和简单可调的外部螺旋阀阵列来完成的。液滴混合或液滴链的可编程序列可以用该设备在低Hz频率下实现，受设备弹性的限制，并可以通过阀门集成进一步增强。该芯片已被用于表征出口过程中的液滴聚集和DNA文库的合成。
We here present and characterize a programmable nanoliter scale droplet-on-demand device that can be used separately or readily integrated into low cost single layer rapid prototyping microfluidic systems for a wide range of user applications. The passive microfluidic device allows external (off-the-shelf) electronically controlled pinch valves to program the delivery of nanoliter scale aqueous droplets from up to 9 different inputs to a central outlet channel. The inputs can be either continuous aqueous fluid streams or microliter scale aqueous plugs embedded in a carrier fluid, in which case the number of effective input solutions that can be employed in an experiment is no longer strongly constrained (100 s-1000 s). Both nanoliter droplet sequencing output and nanoliter-scale droplet mixing are reported with this device. Optimization of the geometry and pressure relationships in the device was achieved in several hardware iterations with the support of open source microfluidic simulation software and equivalent circuit models. The requisite modular control of pressure relationships within the device is accomplished using hydrodynamic barriers and matched resistance channels with three different channel heights, custom parallel reversible microfluidic I/O connections, low dead-volume pinch valves, and a simply adjustable array of external screw valves. Programmable sequences of droplet mixes or chains of droplets can be achieved with the device at low Hz frequencies, limited by device elasticity, and could be further enhanced by valve integration. The chip has already found use in the characterization of droplet bunching during export and the synthesis of a DNA library.