Bioluminescent Probe for Hydrogen Peroxide Imaging in Vitro and in Vivo
Bioluminescent Probe for Hydrogen Peroxide Imaging in Vitro and in Vivo
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DOI：
10.1021/ac502396g
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发表时间：
2014-10-07
影响因子：
7.4
通讯作者：
Li, Minyong
中科院分区：
文献类型：
--
作者：
Wu, Wenxiao;Li, Jing;Li, Minyong
活性氧（reactive oxygen species，ROS）在介导氧化还原修饰和其他重要的生理过程如生物过程调节和信号转导中具有重要作用。考虑到H2O2是ROS的重要成员，H2O2的检测和定量承担着重要而紧迫的责任。本文设计、合成了一种用于检测H2O2的生物荧光探针。该探针被设计成三个部分：H2O2敏感的芳基硼酸，生物发光的氨基荧光素部分，和一个自分解的接头。经过广泛的评价，该探针可以选择性和灵敏度与H2O2反应，释放氨基荧光素。应该指出的是，这种探针是一个潜在的生物发光传感器H2O2，因为它可以提供一个有前途的工具包，用于实时检测H2O2水平在体外，在cellulo，和在体内。
Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H2O2 is a substantial member of ROS, detection and quantitation of H2O2 undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H2O2 was well designed, synthesized, and evaluated. This probe was designed into three parts: a H2O2-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H2O2 to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H2O2 since it can provide a promising toolkit for real-time detection of the H2O2 level in vitro, in cellulo, and in vivo.