Isolation and characterization of microsatellite loci from Larix kaempferi
Isolation and characterization of microsatellite loci from Larix kaempferi
复制标题
DOI：
10.1111/j.1471-8286.2006.01291.x
复制
发表时间：
2006-09-01
期刊：
影响因子：
--
通讯作者：
Watanabe, Atsushi
中科院分区：
文献类型：
--
作者：
Isoda, Keiya;Watanabe, Atsushi
对日本落叶松（Larix kaempferi）的微卫星进行了分离和鉴定。利用殖民地聚合酶链反应（PCR）技术，对富含（AG）（n）重复序列的落叶松基因组DNA文库进行了筛选，获得了145个微卫星序列。共设计了72对引物，其中30对产生单位点扩增产物，19对具有多态性。期望杂合度范围为0.566 - 0.951。这19个多态性微卫星位点可作为日本落叶松遗传研究的重要标记。
Microsatellites were isolated and characterized for Japanese larch, Larix kaempferi, a conifer species distributed in Japan. A larch genomic DNA library enriched for (AG)(n) repeats was screened using the colony polymerase chain reaction method and 145 unique microsatellite containing sequences were obtained. Seventy-two primer pairs were designed and 30 produced single-locus products, and 19 of them were polymorphic. The expected heterozygosity ranged from 0.566 to 0.951. These 19 polymorphic microsatellite loci should be valuable markers for genetic studies on Japanese larch.