Self-organized centripetal movement of corneal epithelium in the absence of external cues.
Self-organized centripetal movement of corneal epithelium in the absence of external cues.
复制标题
DOI：
10.1038/ncomms12388
复制
发表时间：
2016-08-08
影响因子：
16.6
通讯作者：
Lyons JG
中科院分区：
文献类型：
--
作者：
Lobo EP;Delic NC;Richardson A;Raviraj V;Halliday GM;Di Girolamo N;Myerscough MR;Lyons JG
维持角膜的结构对于高质量的视力至关重要。在成年哺乳动物中，角膜上皮细胞起源于利姆布斯中的干细胞，由未知机制驱动朝向角膜中心作为粘性克隆群。在这里，我们使用互补的数学和生物学模型来表明，角膜上皮细胞可以自组织成一个有凝聚力的，向心生长模式，在没有外部生理线索。需要三个条件：干细胞的圆周位置，有限数量的细胞分裂和响应群体压力的移动性。我们已经使用这些互补模型来解释由创伤引起的向心迁移率增加和干细胞渗漏的可能性，以解释来自中央角膜组织的稳定移植，尽管干细胞主要位于角膜缘。 角膜由起源于干细胞外圈并向其中心移动的细胞形成。在这里，作者表明，运动模式是自组织的，不需要任何线索，干细胞渗漏可能是角膜中心干细胞存在的原因。
Maintaining the structure of the cornea is essential for high-quality vision. In adult mammals, corneal epithelial cells emanate from stem cells in the limbus, driven by an unknown mechanism towards the centre of the cornea as cohesive clonal groups. Here we use complementary mathematical and biological models to show that corneal epithelial cells can self-organize into a cohesive, centripetal growth pattern in the absence of external physiological cues. Three conditions are required: a circumferential location of stem cells, a limited number of cell divisions and mobility in response to population pressure. We have used these complementary models to provide explanations for the increased rate of centripetal migration caused by wounding and the potential for stem cell leakage to account for stable transplants derived from central corneal tissue, despite the predominantly limbal location of stem cells. The cornea is formed of cells that originate from the outer circle of stem cells and that move towards its centre. Here, the authors show that the movement pattern is self-organised, requiring no cues, and that stem cell leakage may account for the presence of stem cells at the centre of the cornea.