Induction of supernumerary primordial germ cells by injecting vegetal pole cytoplasm into Xenopus eggs
Induction of supernumerary primordial germ cells by injecting vegetal pole cytoplasm into Xenopus eggs
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将植物极细胞质注射到爪蟾卵中诱导多余的原始生殖细胞
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
M. Wakahara
中科院分区：
文献类型：
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作者：
M. Wakahara
将来自非洲爪蟾卵植物极细胞质的一部分以 20,000 xG 沉淀，当注射到受精卵植物极附近的皮层下细胞质中时，能够诱导多余的原始生殖细胞 (PGC)。用链霉蛋白酶（链霉蛋白酶 E，200 μg/ml，37°C 30 分钟）消化该级分，并没有消除诱导多余 PGC 的能力。讨论了“生发决定因素”的可能性质。
A fraction, precipitated at 20,000 xG, from vegetal pole cytoplasm of Xenopus laevis eggs was able to induce supernumerary primordial germ cells (PGCs) when injected into subcortical cytoplasm near the vegetal pole of the fertilized egg. The ability to induce supernumerary PGCs was not abolished by digesting the fraction with pronase (Pronase E, 200 μg/ml, at 37° C for 30 min). The possible nature of the “germinal determinant” is discussed.