Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells.
Enhancement of individual differences in proliferation and differentiation potentials of aged human adipose-derived stem cells.
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DOI：
10.1016/j.reth.2016.12.004
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发表时间：
2017-06
影响因子：
4.3
通讯作者：
Akamatsu H
中科院分区：
文献类型：
--
作者：
Kawagishi-Hotta M;Hasegawa S;Igarashi T;Yamada T;Takahashi M;Numata S;Kobayashi T;Iwata Y;Arima M;Yamamoto N;Yagami A;Nakata S;Uzawa T;Matsunaga K;Sugiura K;Akamatsu H
脂肪源性干细胞（ASC）是一种强大的多能细胞来源。它们很容易获得，在许多再生应用中有希望。一般认为，体干细胞的功能随着年龄的增长而下降。虽然一些研究已经检查了供体年龄对ASCs增殖潜力和多能性的影响，但这些研究的结果并不一致。本研究测试了供体年龄是否影响脂肪组织中ASCs的产量，以及ASCs的增殖和分化潜力。本研究使用了从260名供体（年龄5 - 97岁）的脂肪组织中获得的ASC。在体外检查ASCs增殖、成脂、成骨和成软骨分化潜能的个体差异。通过主成分分析（PCA），使用其潜在的参数，从每个供体的ASCs的特性进行了评价。对ASC的分析表明，成脂潜力随着年龄的增长而下降，但增殖、成骨和成软骨潜力与年龄无关。有趣的是，在所有的ASC潜力，包括脂肪形成，观察到个体差异。主成分分析（PCA）显示，在老年人的个体差异变得明显，这些变化在女性比男性更突出。这项研究证明了ASCs电位的年龄相关变化，并揭示了ASCs的个体差异在60岁以上的人中变得显着（对于60岁以上的女性和80岁以上的男性）。我们认为，仔细观察ASC的潜力是很重要的，以便使用ASC实现有效的再生医学治疗。ASC可以从所有年龄的受试者中分离。ASCs的成脂潜力随年龄增长而下降。ASCs的成软骨和成骨潜能不受年龄的影响。ASCs的增殖和分化潜能是不同的。60岁以后，ASC电位的个体差异变得显著。
Adipose-derived stem cells (ASCs) are a robust, multipotent cell source. They are easily obtained and hold promise in many regenerative applications. It is generally considered that the function of somatic stem cells declines with age. Although several studies have examined the effects of donor age on proliferation potential and pluripotency of ASCs, the results of these studies were not consistent. This study tested whether the donor age affects the yield of ASCs from adipose tissue, as well as the proliferation and differentiation potentials of ASCs. This study used ASCs obtained from adipose tissues of 260 donors (ages 5–97 years). ASCs were examined for individual differences in proliferation, and adipogenic, osteogenic and chondrogenic differentiation potentials in vitro. Characteristics of ASCs from each donor were evaluated by the principal component analysis (PCA) using their potential parameters. Analyses on ASCs demonstrated that adipogenic potentials declined with age, but proliferation, osteogenic and chondrogenic potentials were not correlated with age. Interestingly, in all ASC potentials, including adipogenesis, individual differences were observed. Principal component analysis (PCA) revealed that individual differences became evident in the elderly, and those variations were more prominent in females than in males. This study demonstrated age-related changes in the potentials of ASCs and revealed that the individual differences of ASCs become significant in people over 60 years of age (for females over 60, and for males over 80). We believe that it is important to carefully observe ASC potentials in order to achieve effective regenerative medicine treatments using ASCs. ASCs can be isolated from subjects in all ages. Adipogenic potential of ASCs declines with age. Chondrogenic and osteogenic potentials of ASCs are not affected by age. Proliferation and differentiation potentials of ASCs are individually different. Individual difference of ASC potentials becomes significant over 60 years of age.