Local site preference rationalizes disentangling by DNA topoisomerases.
Local site preference rationalizes disentangling by DNA topoisomerases.
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DOI：
10.1103/physreve.81.031902
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发表时间：
2010-03
期刊：
影响因子：
--
通讯作者：
Chan HS
中科院分区：
文献类型：
--
作者：
Liu Z;Zechiedrich L;Chan HS
为了合理化的解缠行动的II型拓扑异构酶，一个改进的蠕虫状DNA模型被用来描绘的程度unknotting和decatenating可实现的选择性片段通过在特定的并置几何形状，并确定这些活动是如何受到影响的DNA圈的大小和溶液离子强度。我们发现，在钩状几何形状的节段通道可以减少结人口显着在实验中看到的。选择性片段通道也提供了一个有趣的经验之间的比例关系的理论基础解结和decatenating潜力。
To rationalize the disentangling action of type II topoisomerases, an improved wormlike DNA model was used to delineate the degree of unknotting and decatenating achievable by selective segment passage at specific juxtaposition geometries and to determine how these activities were affected by DNA circle size and solution ionic strength. We found that segment passage at hooked geometries can reduce knot populations as dramatically as seen in experiments. Selective segment passage also provided theoretical underpinning for an intriguing empirical scaling relation between unknotting and decatenating potentials.