Simple, Rapid and Effective Separation of Nuclear Red Blood Cells from Peripheral Blood of Pregnant Women: Preliminary Study
Simple, Rapid and Effective Separation of Nuclear Red Blood Cells from Peripheral Blood of Pregnant Women: Preliminary Study
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简单、快速、有效地从孕妇外周血中分离核红细胞：初步研究
DOI：
10.26717/bjstr.2018.03.000907
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. Kantake
中科院分区：
文献类型：
--
作者：
M. Kantake
孕妇核红细胞（NRBC）分析被认为是一种实用的、非侵入性的产前检查，对了解产前胎儿状态非常有用。在这里，我们展示了一种简单，快速，有效的方法来分离NRBC从孕妇外周血早产。我们使用了MACS微珠技术，该技术基于MACS微珠、与超顺磁性50 nm颗粒偶联的高度特异性抗体、含有铁磁球基质的MACS色谱柱和提供强磁场的MACS分离器。该方法可将CD 45-CD 71+细胞从全血细胞的0.3%富集到分离细胞的93%。当这些分离的细胞中胎儿细胞的比例在一定程度上较高时，我们的方法可能有助于进一步分析。该方法从2 ml母血中获得的NRBC不仅可用于靶向DNA甲基化分析，还可用于下一代测序仪的全外显子组和全转录组分析，这可能会导致围产期医学的进步。
The analysis of nuclear red blood cells (NRBCs) in pregnant woman is believed to be a practical, noninvasive prenatal test that is much useful for understanding the prenatal fetal status. Here, we show a simple, rapid and effective method to separate NRBC from maternal peripheral blood with preterm labor. We used MACS Technology which is based on MACS MicroBeads, highly specific antibodies coupled to super paramagnetic 50nm particles, MACS Columns containing a matrix of ferromagnetic spheres, and MACS Separators providing a strong magnetic field. This method could enrich the CD45-CD71+ cells from 0.3% of whole blood cells to 93% of separated cells. When the fetal cell ratio in these separated cells is high in some extent, our method may be useful for further analysis. The yield of NRBCs from 2ml of maternal blood by this method is enough to be used for not only targeted DNA methylavtion analysis but whole exome and whole transcriptome analysis using next generation sequencer which may lead to progress in perinatology.
影响因子：
20.3
作者：
M. Loken;V. O. Shah;K. Dattilio;C. Civin
通讯作者：
M. Loken;V. O. Shah;K. Dattilio;C. Civin