Caenorhabditis elegans operons contain a higher proportion of genes with multiple transcripts and use 3' splice sites differentially.
Caenorhabditis elegans operons contain a higher proportion of genes with multiple transcripts and use 3' splice sites differentially.
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DOI：
10.1371/journal.pone.0012456
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发表时间：
2010-08-27
期刊：
影响因子：
3.7
通讯作者：
Ma L
中科院分区：
文献类型：
--
作者：
Wang F;Huang S;Ma L
RNA剪接从单个基因产生多个转录异构体，并增强真核基因表达的复杂性。在某些真核生物中，操纵子是一种古老的基因表达调控机制，它要求基因之间有严格的位置关系和调控关系。操纵子基因是否以与非操纵子基因类似的方式产生转录本异构体仍然是未知的，其表达不太可能受到其位置和与周围基因的关系的限制。我们分析了秀丽隐杆线虫操纵基因的转录异构体的数量，发现秀丽隐杆线虫操纵基因的转录异构体的数量与线虫操纵基因的转录异构体的数量有关。与非操纵子基因相比，线虫操纵子含有更高比例的具有多种转录物同种型的基因。对于表达多种转录物同种型的基因，操纵子基因和非操纵子基因中同种型的数目之间没有明显差异。C.与非操纵子基因相比，操纵子基因对20个最常见的3′剪接位点也具有不同的偏好。我们的分析表明C.线虫操纵子通过增加表达多种转录异构体的基因的比例来增强表达复杂性，并通过共同的3′剪接位点的差异使用来维持剪接效率。
RNA splicing generates multiple transcript isoforms from a single gene and enhances the complexity of eukaryotic gene expression. In some eukaryotes, operon exists as an ancient regulatory mechanism of gene expression that requires strict positional and regulatory relationships among its genes. It remains unknown whether operonic genes generate transcript isoforms in a similar manner as non-operonic genes do, the expression of which is less likely limited by their positions and relationships with surrounding genes. We analyzed the number of transcript isoforms of Caenorhabditis elegans operonic genes and found that C. elegans operons contain a much higher proportion of genes with multiple transcript isoforms than non-operonic genes do. For genes that express multiple transcript isoforms, there is no apparent difference between the number of isoforms in operonic and non-operonic genes. C. elegans operonic genes also have a different preference of the 20 most common 3′ splice sites compared to non-operonic genes. Our analyses suggest that C. elegans operons enhance expression complexity by increasing the proportion of genes that express multiple transcript isoforms and maintain splicing efficiency by differential use of common 3′ splice sites.
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DOI：
10.1093/bfgp/3.3.199
发表时间：
2004-11-01
期刊：
Briefings in Functional Genomics & Proteomics
影响因子：
--
作者：
Blumenthal, Thomas
通讯作者：
Blumenthal, Thomas
影响因子：
4.5
作者：
Hollins, C;Zorio, DAR;Blumenthal, T
通讯作者：
Blumenthal, T
影响因子：
64.8
作者：
Wang, Eric T.;Sandberg, Rickard;Luo, Shujun;Khrebtukova, Irina;Zhang, Lu;Mayr, Christine;Kingsmore, Stephen F.;Schroth, Gary P.;Burge, Christopher B.
通讯作者：
Burge, Christopher B.
影响因子：
30.8
作者：
Pan, Qun;Shai, Ofer;Blencowe, Benjamin J.
通讯作者：
Blencowe, Benjamin J.
影响因子：
7
作者：
Kent, WJ;Zahler, AM
通讯作者：
Zahler, AM