Two Arabidopsis cyclin A3s possess G1 cyclin-like features
Two Arabidopsis cyclin A3s possess G1 cyclin-like features
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DOI：
10.1007/s00299-010-0817-9
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发表时间：
2010-02
影响因子：
6.2
通讯作者：
I. Takahashi;S. Kojima;N. Sakaguchi;C. Umeda-Hara;M. Umeda
中科院分区：
文献类型：
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作者：
I. Takahashi;S. Kojima;N. Sakaguchi;C. Umeda-Hara;M. Umeda
A型细胞周期蛋白（CYCA）是一类有丝分裂细胞周期蛋白，与细胞周期蛋白B密切相关。植物CYCA可分为3个亚型（CYCA 1-CYCA 3），其中CYCA 3在G1-S期表达偏性。CYCA 3; 1和CYCA 3; 2转录本在G1/S期高度积累，而CYCA 3; 4在细胞周期中持续表达。CYCA 3; 1和CYCA 3; 2在根、茎顶端分生组织和侧根原基等分裂活跃的组织中表达。CYCA 3; 1或CYCA 3; 2的过表达改变了拟南芥的顶端优势，表明它们在茎分生组织中具有重要的功能。在昆虫细胞中，CYCA 3; 1与CDKA形成活性激酶复合物;1是酵母Cdc 2/Cdc 28 p的直向同源物，磷酸化视网膜母细胞瘤相关蛋白是G1期向S期转变的关键调节因子。我们的研究结果表明，拟南芥CYCA 3; 1和CYCA 3; 2是G1细胞周期蛋白家族的不同成员，在分生组织中发挥重要作用。
A-type cyclins (CYCAs) are a type of mitotic cyclin and are closely related to cyclin B. Plant CYCAs are classified into three subtypes (CYCA1–CYCA3), among whichCYCA3has been suggested to show a biased expression during the G1-to-S phase. We characterisedArabidopsisCYCA3s (CYCA3;1–CYCA3;4) in terms of expression pattern and protein function.CYCA3;1andCYCA3;2transcripts were highly accumulated at the G1/S phase, whereasCYCA3;4was constantly expressed during the cell cycle. Expressions ofCYCA3;1andCYCA3;2were observed in actively dividing tissues, such as root and shoot apical meristems and lateral root primordia. Overexpression ofCYCA3;1orCYCA3;2distorted apical dominance inArabidopsis, indicating that they have critical functions in shoot meristems. In insect cells,CYCA3;1formed an active kinase complex withCDKA;1, an orthologue of the yeast Cdc2/Cdc28p, and phosphorylated retinoblastoma-related protein, a key regulator in the transition from the G1 to the S phase. Our results suggest thatArabidopsisCYCA3;1andCYCA3;2are distinct members of the G1 cyclin family that play an important role in meristematic tissues.