Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay.
Perfluoroalkyl Acid Concentrations in Blood Samples Subjected to Transportation and Processing Delay.
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DOI：
10.1371/journal.pone.0137768
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Nohr EA
中科院分区：
文献类型：
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作者：
Bach CC;Henriksen TB;Bossi R;Bech BH;Fuglsang J;Olsen J;Nohr EA
在对全氟烷基酸的研究中，所测浓度的有效性和可比性可能受到生物标本处理方式差异的影响。我们的目的是研究处理前延迟和运输的血液样本与立即处理和冷冻的血液样本之间的血浆全氟烷基酸水平是否存在差异。在丹麦奥胡斯大学医院招募的孕妇（n = 88）提供了配对的血液样本。对于每一对样本，一个立即处理并冷冻血浆，另一个在处理和冷冻血浆之前延迟并作为全血运输（类似于丹麦国家出生队列）。我们测量了12种全氟烷基酸，并给出了超过50%的样品超过定量下限的化合物的结果。对于冬季采集的样本，配对样本之间的相对差异在个别全氟烷基酸的- 77%至+38%之间。在大多数情况下，延迟和运输样品的浓度较低，例如，全氟辛烷磺酸的相对差异为-29%（95%置信区间为-30；-27）。对于全氟辛酸，两种设置之间没有差异[相应的估计为1%(0,3)]。所有化合物在夏季的差异可以忽略不计。血液样本的运输和处理的延迟，类似于在一些基于人群的大型研究中应用的条件，可能会影响所测量的全氟烷基酸浓度，主要是在室外温度较低的情况下。在人体接触全氟烷基酸的研究中，需要注意加工条件。
In studies of perfluoroalkyl acids, the validity and comparability of measured concentrations may be affected by differences in the handling of biospecimens. We aimed to investigate whether measured plasma levels of perfluoroalkyl acids differed between blood samples subjected to delay and transportation prior to processing and samples with immediate processing and freezing. Pregnant women recruited at Aarhus University Hospital, Denmark, (n = 88) provided paired blood samples. For each pair of samples, one was immediately processed and plasma was frozen, and the other was delayed and transported as whole blood before processing and freezing of plasma (similar to the Danish National Birth Cohort). We measured 12 perfluoroalkyl acids and present results for compounds with more than 50% of samples above the lower limit of quantification. For samples taken in the winter, relative differences between the paired samples ranged between -77 and +38% for individual perfluoroalkyl acids. In most cases concentrations were lower in the delayed and transported samples, e.g. the relative difference was -29% (95% confidence interval -30; -27) for perfluorooctane sulfonate. For perfluorooctanoate there was no difference between the two setups [corresponding estimate 1% (0, 3)]. Differences were negligible in the summer for all compounds. Transport of blood samples and processing delay, similar to conditions applied in some large, population-based studies, may affect measured perfluoroalkyl acid concentrations, mainly when outdoor temperatures are low. Attention to processing conditions is needed in studies of perfluoroalkyl acid exposure in humans.