Development of chemiluminescence detection of gold nanoparticles in biological conjugates for immunoassay
Development of chemiluminescence detection of gold nanoparticles in biological conjugates for immunoassay
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DOI：
10.1016/j.aca.2005.07.014
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发表时间：
2005-10
影响因子：
6.2
通讯作者：
Zhengping Li;Yu-cong Wang;Chenghui Liu;Yan‐kun Li
中科院分区：
文献类型：
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作者：
Zhengping Li;Yu-cong Wang;Chenghui Liu;Yan‐kun Li
以人免疫球蛋白G（IgG）、羊抗人IgG和兔抗羊IgG功能化金纳米颗粒为免疫模型，建立了生物结合物中金纳米颗粒的化学发光检测方法。该方法是基于AuCl 4 −-鲁米诺-H2 O2体系的化学发光反应和荧光免疫反应。AuCl 4 −是金纳米颗粒标记到兔抗羊IgG上的溶解产物，在CL反应中用作分析物，用于间接测量羊抗人IgG。在5 ~ 10μgml-1范围内，化学发光强度与羊抗人IgG浓度的对数成正比，检出限（3σ）为1.5ngml-1。该方法已成功地应用于羊抗人IgG免疫血清样品的测定。作为一种新的化学发光检测方法，标记纳米粒子在生物分析中显示出巨大的潜力。
By using human immunoglobulin G (IgG), goat-anti-human IgG and rabbit-anti-goat IgG-functionalized gold nanoparticles as an immunoassay model, a chemiluminescence (CL) detection of gold nanoparticles in biological conjugates has been developed. The assay is based on the sandwich-type immunoreaction coupled with CL reaction of AuCl4−–luminol–H2O2system. The AuCl4−, which is the dissolving product of the gold nanoparticles labeled to the rabbit-anti-goat IgG, served as an analyte in the CL reaction for the indirect measurement of goat-anti-human IgG. The chemiluminescence intensity is proportional to the logarithm of the concentration of goat-anti-human IgG in the range of 5ngml−1to 10μgml−1and the detection limit (3σ) is 1.5ngml−1. This method has been successfully applied to the determination of goat-anti-human IgG immune serum samples. As a new chemiluminescence detection method, it shows significant potential for the determination of biological analytes by labeling nanoparticles.