Expression and Prognostic Significance of lncRNA MALAT1 in Pancreatic Cancer Tissues
Expression and Prognostic Significance of lncRNA MALAT1 in Pancreatic Cancer Tissues
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DOI：
10.7314/apjcp.2014.15.7.2971
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发表时间：
2014-01-01
影响因子：
--
通讯作者：
Luo, Dian-Zhong
中科院分区：
文献类型：
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作者：
Liu, Jiang-Hua;Chen, Gang;Luo, Dian-Zhong
背景：长链非编码RNA（lncRNA）最近在多种人类癌症中被观察到。然而，lncRNA在胰腺导管腺癌（PDAC）中的作用仍不清楚。本研究的目的是检测lncRNA MALAT 1在PDAC福尔马林固定、石蜡包埋（FFPE）组织中的表达，并探讨其临床意义。研究方法：使用qRT-PCR在45个PDAC和25个邻近的非癌性FFPE组织中以及在5个PDAC细胞系和正常胰腺上皮细胞系HPDE 6c-7中检查MALAT 1的表达。采用Kaplan-Meier法和考克斯比例风险模型分析MALAT 1水平与PDAC临床病理参数的关系。结果：MALAT 1在PDAC中的相对水平显著高于邻近的正常胰腺组织（p=0.009）。当比较培养的细胞系中MALAT 1的水平时，发现MALAT 1在aspc-1 PDAC细胞中的表达显著高于永生胰腺导管上皮细胞系HPDE 6c-7（q=7.573，p
Background: Long non-coding RNAs (lncRNAs) have been recently observed in various human cancers. However, the role of lncRNAs in pancreatic duct adenocarcinoma (PDAC) remains unclarified. The aim of this study was to detect the expression of lncRNA MALAT1 in PDAC formalin-fixed, paraffin embedded (FFPE) tissues and to investigate the clinical significance of the MALAT1 level. Methods: The expression of MALAT1 was examined in 45 PDAC and 25 adjacent non-cancerous FFPE tissues, as well as in five PDAC cell lines and a normal pancreatic epithelium cell line HPDE6c-7, using qRT-PCR. The relationship between MALAT1 level and clinicopathological parameters of PDAC was analyzed with the Kaplan-Meier method and Cox proportional hazards model. Results: The relative level of MALAT1 was significantly higher in PDAC compared to the adjacent normal pancreatic tissues (p=0.009). When comparing the MALAT1 level in the cultured cell lines, remarkably higher expression of MALAT1 was found in aspc-1 PDAC cells compared with the immortal pancreatic duct epithelial cell line HPDE6c-7 (q=7.573, p