Comparative characterization of three Tetraselmis chui (Chlorophyta) strains as sources of nutraceuticals
Comparative characterization of three Tetraselmis chui (Chlorophyta) strains as sources of nutraceuticals
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DOI：
10.1007/s10811-021-02675-x
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发表时间：
2022-01-11
影响因子：
3.3
通讯作者：
Bartual, Ana
中科院分区：
文献类型：
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作者：
Oliveira Moser, Gleyci A.;Barrera-Alba, Jose Juan;Bartual, Ana
由于其高脂肪酸含量（干重5-10%），传统上已被用作水产养殖中有价值的营养来源。它们在人类营养保健品生产中的应用在世界范围内不断增长。其中，茶树四螺富含多不饱和脂肪酸（PUFAs），尤其是二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。我们评估了三种T. chui菌株生产这些营养保健品的潜力：模型菌株CCAP 8/6，因其高PUFA含量而广泛用于水产养殖业，以及两种菌株（TCBG-1和TCBG-2），分离自瓜纳巴拉湾（里约热内卢de Janeiro，巴西）。两株巴西菌株生长速度快于CCAP 8/6， pufa含量较高（指数生长期可达总FA的70%）。它们还产生了大量独特的脂肪酸：TCBG-1在指数阶段产生花生四烯酸（ARA）和EPA(>占总FA的20%)，而TCBG-2在指数后期产生这些PUFAs和DHA（>占总FA的18%）。利用两种巴西菌株在光生物反应器中同时进行规模化培养，提出了一种两阶段生长系统，作为PUFA生产的最佳模型。此外，这两种菌株都适合在热带地区的开放系统中扩大规模，因为它们适应了瓜纳巴拉湾的环境条件，在那里它们通过与其他微藻竞争而形成大规模的水华。
Species of the genus Tetraselmis have traditionally been used as a valuable nutritional source in aquaculture for their high fatty acid content (5-10% dry weight). Their use in nutraceutical production for humans is growing worldwide. Among them, Tetraselmis chui is generally reported as rich in polyunsaturated fatty acids (PUFAs), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). We evaluated the potential of three T. chui strains for the production of these nutraceuticals: the model strain CCAP 8/6, which is broadly used in the aquaculture industry due to its high PUFA content, and two strains (TCBG-1 and TCBG-2) isolated from Guanabara Bay (Rio de Janeiro, Brazil). The two Brazilian strains grew faster than CCAP 8/6 with higher percentage of PUFAs (up to 70% of total FA at the exponential growth phase). They also produced unique fatty acids in significant quantities: TCBG-1 produced arachidonic acid (ARA) and EPA during the exponential phase (> 20% of total FA), while TCBG-2 produced these PUFAs in addition to DHA (> 18% of total FA) at the late exponential phase. A two-stage growth system using co-cultures of the two Brazilian strains is proposed as an optimal model for PUFA production, based on their simultaneous scaling cultivation in photobioreactors. Furthermore, both strains are suitable candidates for upscaling in open systems in tropical regions since they are adapted to the environmental conditions in Guanabara Bay, where they form massive blooms by outcompeting other microalgae.