Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures
Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures
复制标题
丹参毛状根培养物中诱导子诱导丹参酮积累的分子机制
DOI：
10.1007/s11738-012-0940-z
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发表时间：
2012-07-01
影响因子：
2.6
通讯作者：
Zhang, Lin
中科院分区：
文献类型：
--
作者：
Kai, Guoyin;Liao, Pan;Zhang, Lin
丹参酮主要用于心脑血管疾病的治疗，为了开发最佳的丹参酮生物合成工艺，必须更好地了解丹参酮生物合成途径的调控。本文研究了丹参毛状根在生物和非生物激发子诱导下，丹参酮生物合成途径相关基因的表达及丹参酮的积累。结果表明丹参酮在S.丹参毛状根中丹参酮生物合成途径中多个基因的表达受到高度调控。我们的研究结果表明，基因表达和丹参酮积累之间的正相关性，丹参酮的积累可能是在各种诱导子的处理下，参与丹参酮生物合成的几个基因的共表达上调的结果。同时，SmHMGR、SmDXS2、SmFPPS、SmGGPPS和SmCPS被认为是丹参酮代谢途径中潜在的关键酶。丹参毛状根。这是第一次全面系统地研究丹参酮在S.丹参毛状根。
To develop an optimal bioprocess for the production of tanshinone which is mainly used for the treatment of cardiocerebral vascular disease, the tanshinone biosynthetic pathway regulation must be better understood. In this paper, expression of tanshinone biosynthetic pathway related genes as well as tanshinone accumulation in Salvia miltiorrhiza hairy root cultures were investigated, in response to biotic and abiotic elicitors, respectively. Our results showed tanshinone accumulation in S. miltiorrhiza hairy roots was highly regulated by the coordination of the expression of several genes involved in tanshinone biosynthesis pathway. Our results showed a positive correlation between gene expression and tanshinone accumulation, suggesting that tanshinone accumulation may be the result of the coexpression up-regulation of several genes involved in tanshinone biosynthesis under the treatment of various elicitors. Meantime, SmHMGR, SmDXS2, SmFPPS, SmGGPPS and SmCPS were identified as the potential key enzymes in the pathway for targeted metabolic engineering to increase accumulation of tanshinone in S. miltiorrhiza hairy roots. This is the first report integrating comprehensively the transcript and metabolite biosynthesis of tanshinone in S. miltiorrhiza hairy roots.