Human bocavirus in children hospitalized for acute gastroenteritis: A case-control study
Human bocavirus in children hospitalized for acute gastroenteritis: A case-control study
复制标题
DOI：
10.1086/589244
复制
发表时间：
2008-07-15
影响因子：
11.8
通讯作者：
Fang, Zhao-yin
中科院分区：
文献类型：
--
作者：
Cheng, Wei-xia;Jin, Yu;Fang, Zhao-yin
背景人类博卡病毒（humanbocavirus，HBoV）是近年来在儿童呼吸道疾病和胃肠炎中发现的一种病毒。HBoV在人类胃肠炎中的致病作用仍然不确定，据我们所知，以前没有病例对照研究研究HBoV和胃肠炎之间的关系。我们进行了一项病例对照研究，检查了397名腹泻儿童和115名无症状对照受试者的粪便样本。采用聚合酶链反应检测HBoV。使用实时聚合酶链反应来量化病例组和对照组中的HBoV载量。采用酶联免疫吸附试验、聚合酶链反应和逆转录聚合酶链反应检测常见肠道病毒。在60.2%的病例中发现至少1种病毒因子。在14个样本中检测到HBoV，并且9个与轮状病毒（7/14个样本）或人杯状病毒（2/14）共感染。2006年9月至12月期间发生了许多（14例中的8例[57.1%]）HBoV感染。大多数（12 [85.7%]）HBoV感染的儿童年龄为7-18个月。在病例组和对照组之间，HBoV感染儿童的百分比没有显著差异（3.5%对3.5%），统计分析不支持HBoV感染与更严重的临床症状之间的相关性。两组之间的病毒载量差异无统计学意义（P = . 09，通过对数正态Student t检验）。此外，病例组和对照组的HBoV VP 1/VP 2部分基因序列变异较小。在中国人中揭示了HBoV的单一遗传谱系。尽管其在粪便样本中的高流行率，但我们的研究不支持HBoV在胃肠炎中的致病作用。
Background. Human bocavirus (HBoV) was recently discovered in children with respiratory tract disease and gastroenteritis. The causative role of HBoV in human gastroenteritis remains uncertain, and, to our knowledge, no previous case-control study has studied the relationship between HBoV and gastroenteritis.Methods. We conducted a case-control study that examined stool samples from 397 children with diarrhea and from 115 asymptomatic control subjects. HBoV was detected using polymerase chain reaction. Real-time polymerase chain reaction was used to quantify the HBoV loads in case and control groups. Common enteric viruses were examined using enzyme-linked immunosorbent assays, polymerase chain reaction, and reverse-transcription polymerase chain reaction.Results. At least 1 viral agent was discovered in 60.2% of cases. HBoV was detected in 14 samples, and 9 were coinfected with either rotavirus (7 of 14 samples) or human calicivirus (2 of 14). Many (8 [57.1%] of 14) of the HBoV infections occurred during September-December 2006. Most (12 [85.7%]) of the HBoV-infected children were 7-18 months of age. The percentage of children with HBoV infection did not differ significantly between case patients and control subjects (3.5% vs. 3.5%), and the statistical analysis did not support a correlation between HBoV infection and more-severe clinical symptoms. The viral load differences between the 2 groups were not statistically significant (P = . 09, by log-normal Student's t test). In addition, the VP1/VP2 partial gene of HBoV from case patients and control subjects showed minimal sequence variation.Conclusions. A single genetic lineage of HBoV was revealed in persons in China. Despite its high prevalence in stool samples, our study does not support a causative role of HBoV in gastroenteritis.