The genome sequence of the facultative intracellular pathogen Brucella melitensis
The genome sequence of the facultative intracellular pathogen Brucella melitensis
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DOI：
10.1073/pnas.221575398
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发表时间：
2002-01-08
影响因子：
11.1
通讯作者：
Overbeek, R
中科院分区：
文献类型：
--
作者：
DelVecchio, VG;Kapatral, V;Overbeek, R
羊种布鲁氏菌是一种兼性细胞内细菌病原体，可引起山羊和绵羊流产以及人类马耳他热。B的基因组。Melitensis菌株16 M的核苷酸序列为3，294，935 bp，分布在2，117，144 bp和1，177，787 bp的两条环状染色体上，编码3，197个ORF。通过使用生物信息学套件ERGO，2，487（78%）的ORF被分配功能。两条染色体的复制起点与其他α-变形菌相似。管家基因，包括那些参与DNA复制，转录，翻译，核心代谢和细胞壁生物合成，分布在两条染色体上。不存在I、II和III型分泌系统，但鉴定了编码sec依赖性、sec非依赖性和鞭毛特异性III型、IV型和V型分泌系统以及粘附素、侵袭素和溶血素的基因。B的几个特点。Melitensis基因组与共生苜蓿中华根瘤菌基因组相似。
Brucella, melitensis is a facultative intracellular bacterial pathogen that causes abortion in goats and sheep and Malta fever in humans. The genome of B. melitensis strain 16M was sequenced and found to contain 3,294,935 bp distributed over two circular chromosomes of 2,117,144 bp and 1,177,787 bp encoding 3,197 ORFs. By using the bioinformatics suite ERGO, 2,487 (78%) ORFs were assigned functions. The origins of replication of the two chromosomes are similar to those of other alpha-proteobacteria. Housekeeping genes, including those involved in DNA replication, transcription, translation, core metabolism, and cell wall biosynthesis, are distributed on both chromosomes. Type I, II, and III secretion systems are absent, but genes encoding sec-dependent, sec-independent, and flagella-specific type III, type IV, and type V secretion systems as well as adhesins, invasins, and hemolysins were identified. Several features of the B. melitensis genome are similar to those of the symbiotic Sinorhizobium meliloti.