CRISPR-Cas9 Genome Editing in Human Cell Lines with Donor Vector Made by Gibson Assembly.
CRISPR-Cas9 Genome Editing in Human Cell Lines with Donor Vector Made by Gibson Assembly.
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DOI：
10.1007/978-1-0716-0290-4_20
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发表时间：
2020
影响因子：
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通讯作者：
N. Sahoo;Victoria Cuello;Shreya Udawant;Carl Litif;J. Mustard;M. Keniry
中科院分区：
文献类型：
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作者：
N. Sahoo;Victoria Cuello;Shreya Udawant;Carl Litif;J. Mustard;M. Keniry
CRISPR Cas9 基因组编辑允许研究人员以多种方式修改基因，包括获得删除、表位标记基因座和敲入突变。 CRISPR-Cas9基因组编辑自最初应用以来的6年内已得到广泛应用，但该方法存在修饰效率低、脱靶效应等缺点，需要仔细考虑。获得定制的供体载体也可能既昂贵又耗时。本章详细介绍了克服 CRISPR-Cas9 基因组编辑障碍的策略以及采用该技术的最新进展。
CRISPR Cas9 genome editing allows researchers to modify genes in a multitude of ways including to obtain deletions, epitope-tagged loci, and knock-in mutations. Within 6 years of its initial application, CRISPR-Cas9 genome editing has been widely employed, but disadvantages to this method, such as low modification efficiencies and off-target effects, need careful consideration. Obtaining custom donor vectors can also be expensive and time-consuming. This chapter details strategies to overcome barriers to CRISPR-Cas9 genome editing as well as recent developments in employing this technique.