DENSITY ANALYSIS OF HEPATITIS-C VIRUS PARTICLE-POPULATION IN THE CIRCULATION OF INFECTED HOSTS - IMPLICATIONS FOR VIRUS NEUTRALIZATION OR PERSISTENCE
DENSITY ANALYSIS OF HEPATITIS-C VIRUS PARTICLE-POPULATION IN THE CIRCULATION OF INFECTED HOSTS - IMPLICATIONS FOR VIRUS NEUTRALIZATION OR PERSISTENCE
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DOI：
10.1016/0168-8278(95)80107-3
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发表时间：
1995-04-01
影响因子：
25.7
通讯作者：
KAMADA, T
中科院分区：
文献类型：
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作者：
KANTO, T;HAYASHI, N;KAMADA, T
丙型肝炎病毒在蔗糖中的浮力密度较低，但在丙型肝炎病毒感染中经常观察到高密度的颗粒，为了研究循环丙型肝炎病毒颗粒的特征及其与肝脏疾病进展的关系，我们检测了2名组织学正常的丙型肝炎病毒携带者，20名慢性肝炎患者和5名急性丙型肝炎患者的血清，用抗免疫球蛋白抗体免疫沉淀后获得的上清液进行蔗糖平衡离心，处理后分离的HCV-RNA阳性组分进一步用抗丙型肝炎病毒核心抗体检测免疫沉淀，我们用35%蔗糖离心，根据密度差分离丙型肝炎病毒颗粒群，通过竞争性逆转录和聚合酶链反应测定丙型肝炎病毒群体中高密度和低密度颗粒的比例。慢性感染患者中的C病毒颗粒可分为两个群体：这些免疫球蛋白结合密度高，未结合密度低。严重肝脏炎症患者具有高密度丙型肝炎病毒，其不与抗免疫球蛋白沉淀，但与抗核心丙型肝炎病毒抗体沉淀，因此，丙型肝炎病毒颗粒群由低密度病毒体和高密度免疫复合物和/或核衣壳组成。在慢性肝炎患者中，随着肝病的进展，优势人群由低密度颗粒转移到高密度颗粒。在急性肝炎患者中，观察到丙氨酸氨基转移酶正常化的这种密度变化。因此，随着肝脏疾病或炎症的进展，主要的丙型肝炎病毒群体从病毒体转变为免疫复合物和/或核衣壳。
Hepatitis C virus has a low buoyant density in sucrose, but high-density particles are often observed in hepatitis C virus infection.To investigate the characteristics of circulating hepatitis C virus particles and their association with liver disease progression, we examined sera from two histologically normal hepatitis C virus carriers, 20 chronic hepatitis patients and five acute hepatitis C patients, The supernatants obtained after immunoprecipitation with anti-immunoglobulins antibody were subjected to sucrose equilibrium centrifugation, HCV-RNA positive fractions separated after the treatments were further examined for immunoprecipitation with anti-core hepatitis C virus antibody, We separated hepatitis C virus particle populations according to the density difference on 35% sucrose with centrifugation, The proportions of high and low density particles in hepatitis C virus populations were determined by means of competitive reverse transcription and polymerase chain reaction.Circulating hepatitis C virus particles in chronically infected patients could be separated into two populations: those immunoglobulin-bound with high densities and -unbound with low densities, Patients with severe liver inflammation had high-density hepatitis C virus that did not precipitate with anti-immunoglobulins but with anti-core hepatitis C virus antibodies, Thus, hepatitis C virus particle populations consist of low density virions and high-density immune complexes and/or nucleocapsids. Among the chronic hepatitis patients, the dominant population shifted from low-density to high-density particles with the progression of liver disease. In acute hepatitis patients, this density shift was observed with alanine aminotransferase normalizations.Therefore, the major hepatitis C virus populations change from virion to immune complex and/or nucleocapsid with the progression of liver disease or inflammation.