Murine marrow cellularity and the concept of stem cell competition: geographic and quantitative determinants in stem cell biology
Murine marrow cellularity and the concept of stem cell competition: geographic and quantitative determinants in stem cell biology
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DOI：
10.1038/sj.leu.2403268
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发表时间：
2004-03-01
期刊：
影响因子：
11.4
通讯作者：
Quesenberry, PJ
中科院分区：
文献类型：
--
作者：
Colvin, GA;Lambert, JF;Quesenberry, PJ
在无动于衷的小鼠中，骨髓细胞数量与干细胞数量相关。在未经治疗的小鼠中，通过输注高水平的骨髓细胞，可以获得高水平的长期骨髓移植。为了解决干细胞竞争与“开放空间”的问题，必须了解小鼠骨髓细胞总数以及干细胞和祖细胞的分布。我们在不同品系的小鼠身上测定了这些参数。BALB/c小鼠的细胞总数为530+/-2000万个，从8周到1岁保持稳定。C57BL/6J小鼠有466+/-4800万个骨髓细胞。使用这些数据，输注骨髓(4000万个细胞)替换或添加到宿主骨髓的理论模型给出的嵌合值分别为7.5%和7.0%，观察到4000万个雄性BALB/c骨髓细胞植入雌性宿主(72只小鼠)8周的嵌合值为6.91+/-0.4%。这表明同基因植入是由干细胞竞争决定的。我们的研究表明，大多数骨髓细胞、祖细胞和可植入干细胞都在脊髓中。祖细胞在脊柱中的浓度增加。用于干细胞纯化和其他实验目的的全骨髓收获既具有老鼠的效率，又具有成本效益，动物使用量减少了四倍以上，并节省了资金。
In unperturbed mice, the marrow cell numbers correlate with the stem cell numbers. High levels of long-term marrow engraftment are obtained with infusion of high levels of marrow cells in untreated mice. To address the issue of stem cell competition vs 'opening space', knowledge of total murine marrow cellularity and distribution of stem and progenitor cells are necessary. We determined these parameters in different mouse strains. Total cellularity in BALB/c mice was 530+/-20 million cells; stable from 8 weeks to 1 year of age. C57BL/6J mice had 466+/-48 million marrow cells. Using these data, theoretical models of infused marrow (40 million cells) replacing or adding to host marrow give chimerism values of 7.5 and 7.0%, respectively; the observed 8-week engraftment of 40 million male BALB/c marrow cells into female hosts (72 mice) gave a value of 6.91+/-0.4%. This indicates that syngeneic engraftment is determined by stem cell competition. Our studies demonstrate that most marrow cells, progenitors and engraftable stem cells are in the spine. There was increased concentration of progenitors in the spine. Total marrow harvest for stem cell purification and other experimental purposes was both mouse and cost efficient with over a four-fold decrease in animal use and a financial saving.