IDENTIFICATION OF THE CONSERVED, CONFORMATION-DEPENDENT CYTOKERATIN EPITOPE RECOGNIZED BY MONOCLONAL-ANTIBODY (LU-5)
IDENTIFICATION OF THE CONSERVED, CONFORMATION-DEPENDENT CYTOKERATIN EPITOPE RECOGNIZED BY MONOCLONAL-ANTIBODY (LU-5)
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DOI：
10.1007/bf00712737
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发表时间：
1987-01-01
期刊：
影响因子：
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通讯作者：
STAHLI, C
中科院分区：
文献类型：
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作者：
FRANKE, WW;WINTER, S;STAHLI, C
由鼠单克隆抗体（mAB lu-5）识别的表位最近被描述为在肿瘤诊断中具有重要价值的抗甲氨蝶呤的“泛上皮标记物”，其位于细胞角蛋白丝的表面上。由于该表位在SDS-聚丙烯酰胺凝胶电泳（SDS-PAGE）后不具有反应性，因此已通过斑点印迹抗体结合试验，使用其中重组表位的纯化蛋白来鉴定具有表位的蛋白。我们表明，表位是存在于大多数细胞角蛋白多肽的酸性（I型）和碱性（II型）亚家族，但不发生在其他细胞骨架蛋白。这个广泛的表位的位置进行了讨论相对于同源性的氨基酸序列的细胞角蛋白和它们的构象。
The epitope recognized by the murine monoclonal antibody (mAB lu-5) recently described as a formaldehyde-resistant, “pan-epithelial marker” of great value in tumour diagnosis is located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution from amphibia to man. As this epitope is not reactive after SDS-polyacrylamide gel electrophoresis (SDS-PAGE), the epitope-bearing protein has been identified by a dot-blot antibody binding assay, using purified proteins in which the epitope is reconstituted. We show that the epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The location of this widespread epitope is discussed with respect to homologies of amino acid sequences of cytokeratins and their conformations.