Systemic toxicity induced by aggregated layered double hydroxide nanoparticles.
Systemic toxicity induced by aggregated layered double hydroxide nanoparticles.
复制标题
聚集的层状双氢氧化物纳米颗粒诱导的全身毒性
DOI：
10.2147/ijn.s146414
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发表时间：
2017
影响因子：
8
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Yan M;Yang C;Huang B;Huang Z;Huang L;Zhang X;Zhao C
层状双氢氧化物（LDH）纳米颗粒是生物医学领域应用前景广阔的纳米材料之一，但其体内系统毒性却很少受到关注。本研究考察了无机纳米颗粒聚集对其全身毒性的影响。值得注意的是，裸LDH纳米颗粒与生理盐水或红细胞混合后观察到聚集。裸体LDH纳米颗粒在静脉给药1 h后在小鼠肺内明显蓄积，重复注射6次后小鼠存活率为0%。死亡小鼠肺泡内出现絮状沉淀，肺间质充血。然而，脂质膜包裹的LDH纳米颗粒不会形成聚集体，并且可以静脉注射bbbb6次而不会造成死亡。这些结果表明，即使在低剂量（30 mg/kg）下重复注射LDH也是致命的，脂质膜涂层可以考虑作为降低这种风险的方法。
Layered double hydroxide (LDH) nanoparticles are emerging as one of the promising nanomaterials for biomedical applications, but their systemic toxicity in vivo has received little attention. In the present study, the effects of inorganic nanoparticle aggregation on their systemic toxicity were examined. Remarkably, aggregation was observed after the mixing of naked LDH nanoparticles with saline or erythrocytes. Significant accumulation of the naked LDH nanoparticles in the lungs of mice was detected 1 h after intravenous administration, and the survival rate of mice was 0% after 6 repeated injections. Furthermore, flocculent precipitates in the alveoli and congestion in the lung interstitium were observed in the dead mice. However, lipid membrane-coated LDH nanoparticles would not form aggregates and could be injected intravenously >6 times without causing death. These findings suggested that repeated injections of LDH were lethal even at low dose (30 mg/kg), and lipid membrane coating can be considered as an approach for reducing this risk.